姜黄素驱动的氧化应激损伤DNA体外抗黑色素瘤的研究  

Curcumin-Induced Oxidative Stress Against Melanoma by DNA Damage:An In Vitro Study

作　　者：赵相宜 晏敏 卢靖宇 冷帆 李童斐 孔存青 ZHAO Xiang-yi;YAN Min;LU Jing-yu;LENG Fan;LI Tong-fei;KONG Cun-qing(School of Basic Medical Sciences,Hubei University of Medicine,Shiyan,Hubei 442000,China;School of Biomedical Engineering,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院生物医学工程学院,湖北十堰442000

出　　处：《湖北医药学院学报》2024年第6期613-617,共5页Journal of Hubei University of Medicine

基　　金：山东省自然科学基金项目(ZR2023QH145);湖北省科技厅项目(2023AFB837);湖北医药学院人才启动金项目(2020QDJZR002,2023QDJZR29,2022QDJZR006)。

摘　　要：目的:验证姜黄素(CUR)能否通过驱动氧化应激诱导黑色素瘤细胞DNA损伤而促进其凋亡并抑制其增殖。方法:利用CCK-8及流式细胞术检测CUR被黑色素瘤细胞摄取的情况及其对黑色素瘤细胞活力的影响;利用蛋白质免疫印迹法、彗星实验等检测CUR对黑色素瘤细胞活性氧生成及DNA损伤的影响。结果:CUR可以被黑色素瘤细胞摄取,且随着浓度增加可以显著降低其细胞活力。CUR可显著提高细胞内活性氧水平,诱导黑色素瘤细胞DNA损伤、细胞凋亡并抑制其增殖。结论:CUR可通过驱动氧化应激损伤黑色素瘤细胞DNA进而发挥抗黑色素瘤作用,为CUR应用于黑色素瘤的治疗提供理论依据。Objective To verify whether curcumin(CUR)-induced oxidative stress promotes apoptosis and inhibit prolifera⁃tion in melanoma cells by inducing DNA damage.Methods The uptake of CUR by melanoma cells and its effect on cell via⁃bility were detected using CCK-8 and flow cytometry.The impacts of CUR on reactive oxygen species(ROS)and DNA damage in melanoma cells were examined using Western blot and comet assay.Results CUR was taken up by melanoma cells,and it significantly reduced the cell viability with increasing concentration.Additionally,CUR remarkably increased the ROS level,leading to DNA damage,apoptosis,and thus proliferation blockade in melanoma cells.Conclusion This study indicates that CUR-induced oxidative stress has anti-melanoma effect by inducing DNA damage,providing a theoret⁃ical basis for the application of CUR in the treatment of melanoma.

关 键 词：黑色素瘤 姜黄素 DNA损伤 活性氧 

分 类 号：R285[医药卫生—中药学]