丙酮酸盐腹膜透析液腹腔注射对感染性休克大鼠腹内脏器的影响  

Effect of Peritoneal Injection of Pyruvate Peritoneal Dialysis Solution on Abdominal Organs in Septic Shock Rats

作　　者：罗兴均 潘雪莲 冉然 孙鸿然 黄立德 LUO Xing-jun;PAN Xue-lian;RAN Ran;SUN Hong-ran;HUANG Li-de(Department of Anesthesiology,Renmin Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]十堰市人民医院·湖北医药学院附属人民医院麻醉科,湖北十堰442000

出　　处：《湖北医药学院学报》2024年第6期627-631,F0003,共6页Journal of Hubei University of Medicine

基　　金：十堰市科学技术研究与开发计划项目(22Y64)。

摘　　要：目的:观察丙酮酸盐腹膜透析液腹腔注射对感染性休克大鼠腹内脏器的影响。方法:54只健康雄性SD大鼠,通过静脉注射内毒素复制感染性休克大鼠模型,将模型复制成功的45只SD大鼠随机均分为单纯静脉复苏组(A组)、静脉复苏+腹腔注射乳酸盐腹膜透析液组(B组)、静脉复苏+腹腔注射丙酮酸盐腹膜透析液组(C组)三组。静脉复苏采用林格氏液25 mL/kg快速静脉输注30 min,随后B组腹腔注入乳酸盐腹膜透析液100 mL/kg,输注时间20 min,C组腹腔注入等量丙酮酸盐腹膜透析液。复苏20 h后统计大鼠存活情况,解剖观察存活大鼠腹内脏器变化,取肝脏、回肠等内脏组织用于测定组织干湿重比、MDA含量、MPO活性及TNF-α表达水平并做组织病理切片HE染色等。结果:B、C组大鼠复苏20 h后存活率高于A组,而C组则高于B组(P<0.05);B、C组大鼠肝脏、回肠组织MDA含量、MPO活性、TNF-α表达水平、组织水肿程度及形态学损伤程度均低于A组,C组则低于B组(P<0.05)。结论:丙酮酸盐腹膜透析液腹腔注射能够抑制感染性休克大鼠腹内脏器脂质过氧化和炎性反应,有效减轻感染性休克大鼠腹内脏器损伤,改善休克治疗效果。Objective To observe the effect of peritoneal injection of pyruvate peritoneal dialysis solution on the abdominal organs in septic shock rats.Methods Fifty-four healthy male SD rats were used to replicate a septic shock model by intra⁃venous injection of endotoxins.The 45 successfully modeled rats were randomly divided into three groups:single intrave⁃nous resuscitation(group A),intravenous resuscitation+peritoneal injection of lactate peritoneal dialysis solution(group B),and intravenous resuscitation+peritoneal injection of pyruvate peritoneal dialysis solution(group C).Intravenous resus⁃citation was performed by rapid intravenous infusion of Ringer′s solution at 25 mL/kg for 30 min,followed by peritoneal in⁃jection of lactate peritoneal dialysis solution at 100 mL/kg for 20 min in group B,or an equal amount of pyruvate peritoneal dialysis solution in group C.After 20 h of resuscitation,the survival status of the rats was recorded.Changes in the abdomi⁃nal organs of the survived rats were observed anatomically,and the liver and ileum tissues were collected to determine the dry wet weight ratio,MDA content,MPO activity,and TNF-α.Histopathological sections were stained with HE.Results The survival rate of rats in groups B and C was higher than that of group A after resuscitation for 20 h,while that of group C was higher than that of group B(P<0.05).The MDA content,MPO activity,TNF-α,tissue edema,and morphological damage in the liver and ileum tissues of rats in groups B and C were lower than those of group A,and group C was lower than group B in the above indexes(P<0.05).Conclusion Peritoneal injection of pyruvate peritoneal dialysis solution in⁃hibits lipid peroxidation and inflammatory reactions in the abdominal organs of septic shock rats and thus reduces the dam⁃age to the abdominal organs,which improves the treatment effectiveness of shock.

关 键 词：丙酮酸盐 腹腔注射 感染性休克 腹内脏器 

分 类 号：R459.7[医药卫生—急诊医学]