机构地区:[1]浙江农林大学园艺科学学院,杭州311300 [2]浙江省农业科学院园艺研究所,杭州310021 [3]浙江省种子管理总站,杭州310021
出 处:《果树学报》2024年第12期2377-2388,共12页Journal of Fruit Science
基 金:浙江省“十四五”果品新品种选育专项(2021C02066-6);浙江省现代种业发展项目(2024R05A60D01);现代农业产业技术体系建设专项(CARS-29-13)。
摘 要:【目的】采用相关性分析、聚类分析与主成分分析法对浙北平原地区101份鲜食葡萄果实品质性状进行综合评价,对其代表性品质进行表征,为葡萄果实品质评价和优良种质筛选提供技术指导。【方法】以101份鲜食葡萄种质资源成熟期果实为试材,对纵径、横径、粒质量、可溶性固形物含量、果实硬度、弹力、总酚含量、类黄酮含量、花色苷含量、总糖含量、总酸含量、裂果率、裂果指数等26个果实品质相关指标进行测定和分析,采用相关性分析、聚类分析与主成分分析进行综合评价。【结果】26个品质性状间具有显著差异,且多项指标存在相关性;通过聚类分析可将101份鲜食葡萄种质资源在欧式距离11.5时分为5个类群;主成分分析可将14个性状归为5个主成分,累计贡献率为82.178%,分别反映葡萄的果实含糖量、营养物质含量、抗裂果能力、果肉质地和风味特征。结合不同评价方式,在浙北平原地区,综合评价结果为蜜光、黑珍珠、郑艳无核、金手指、09-42、黑色甜菜、13-653、15-115等葡萄种质综合品质性状较优,新郁、夜美人、里扎马特相对较差。【结论】不同葡萄种质资源间果实品质指标差异较大,对101份鲜食葡萄种质进行品质评价,筛选出可溶性固形物含量、总糖含量、总酚含量、类黄酮含量、弹力、凝聚性、咀嚼性、糖酸比、裂果率和裂果指数作为葡萄果实品质评价的核心指标,以及8份综合品质优异的葡萄种质资源,可作为浙江地区鲜食葡萄种质资源评价和品种选育鉴定工作的重要参考指标。【Objective】There are various trait differences among different grape germplasms.The as-sessment of fruit characteristics plays a vital role in the assessment of grape genetic resources.The study aimed to comprehensively evaluate the berry quality traits of the 101 table grapes cultivated in the northern Zhejiang Plain using correlation analysis,cluster analysis and principal component analysis,in order to characterize their representative qualities,and provide technical guidance for the evaluation of grape fruit quality and the screening of excellent germplasm.【Methods】A total of 101 table grape germplasm resources were used as test materials to investigate and analyze the fruit quality traits.In or-der to explore the fruit quality traits,26 parameters were measured including vetotal organic acidsticity,cohesion,elastic index,chewiness,pericarp puncture strength,total phenols content,flavonoid content,proanthocyanidin,sucrose content,fructose content,glucose content,oxalic acid content,tartaric acid content,malic acid content,citric acid content,total sugar content,total organic acid content,the ratio of total sugar to total organic acid,fruit cracking rate and fruit cracking index.SPSS 27.0 was used to conduct correlation analysis,cluster analysis,and principal component analysis on the above indicators,and to analyze and evaluate the quality of grape fruits through comprehensive scoring ranking.【Re-sults】The 26 quality indicators showed varying degrees of variation,with coefficients of variation rang-ing from 7.03%to 159.48%.Among all indicators,the proanthocyanidin had the largest variation and the elastic index had the smallest.According to the cluster analysis,the 101 grape germplasm resources were divided into 5 categories when the Euclidean distance was 11.5.TheⅠcategory could be divided into 3 groups when the Euclidean distance was 8.5,the majority were derived from hybridization;theⅡcategory could be divided into 4 groups when the Euclidean distance was 8.5,the majority were Eur-asian pop
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