柑橘热应激转录因子的全基因组鉴定及其在柑橘日灼果皮中的表达特性  

Genome-wide identification of citrus heat stress transcription factors and their expression characteristics in citrus sunburned peel

作　　者：胡亚威 彭淼[1,3] 阳灿 李荣华 刘鑫 陈岳文 杨长耀[1,2,3] 杨金磊 卢嘉伟 卢晓鹏 杨俊枫 HU Yawei;PENG Miao;YANG Can;LI Ronghua;LIU Xin;CHEN Yuewen;YANG Chang-yao;YANG Jinlei;LU Jiawei;LU Xiaopeng;YANG Junfeng(College of Horticulture,Hunan Agricultural University,Changsha 410128,Hunan,China;National Center for Citrus Improvement Changsha,Changsha 410128,Hunan,China;Yuelushan Laboratory,Changsha 410128,Hunan,China;Citrus Science Research Insti-tute of Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan,China;Agriculture and Rural Bureau of Yizhang County of Hunan,Yizhang 424200,Hunan,China)

机构地区：[1]湖南农业大学园艺学院,长沙410128 [2]国家柑橘改良中心长沙分中心,长沙410128 [3]岳麓山实验室,长沙410128 [4]湘西土家族苗族自治州柑橘科学研究所,湖南吉首416000 [5]湖南省宜章县农业农村局,湖南宜章424200

出　　处：《果树学报》2024年第12期2408-2424,共17页Journal of Fruit Science

基　　金：现代农业产业技术体系建设专项(CARS-26);国家自然科学基金(32102313、32172520);湖南省教育厅优秀青年项目(22B0213)。

摘　　要：【目的】在全基因组水平上鉴定柑橘属橙类、宽皮柑橘类、柚类的Hsf家族成员及其在柑橘果实日灼形成和热胁迫中的表达特性,并挖掘可能参与柑橘果实日灼发生过程中的关键Hsf基因。【方法】应用生物信息学方法对柑橘属Hsf家族成员进行鉴定,并对其进化发育、结构特征、顺式作用元件和共线性进行分析;同时还分析了柑橘转录组和日灼果皮不同组织及热处理滑皮金柑果皮中Hsf的表达模式。【结果】从甜橙、克里曼丁橘、晚白柚基因组中分别鉴定出14、20、16个Hsf基因,进化发育分析将其分为HSFA~C3个簇,同一簇成员具有相似且保守的结构特征,片段复制事件在柑橘Hsf家族扩张中起主导作用。茉莉酸甲酯响应元件(TGACG-motif、CGTCA-motif)、脱落酸响应元件(ABRE)及干旱诱导响应元件(MBS)在Hsfs启动子中分布最为广泛。大多数CsHsfs成员在奉节脐橙外果皮或中果皮中特异表达。CsHsf1/5/6/8/11在温州蜜柑日灼与正常果皮之间、热处理与对照金柑果皮之间显著差异表达。尤其是CsHsf5在多个柑橘组织表达谱及温州蜜柑日灼果皮和热处理金柑果皮中均显著上调表达,推测其可能在柑橘果实日灼过程中有重要的功能。【结论】鉴定并分析了柑橘属Hsf成员,明晰了其在日灼果皮及热处理果皮组织中的表达特性,为系统解析柑橘属Hsfs基因功能及柑橘果实日灼分子机制研究提供理论基础。【Objective】The purpose of this study was to identify the members of the Hsf gene family in citrus species,including sweet orange,mandarin,and pomelo at the whole-genome level,and to investi-gate the expression characteristics of CsHsfs in sunburned fruit peel of satsuma mandarin and heat-stressed peel of kumquat,as well as to explore the key Hsf genes possibly involved in citrus fruit sun-burn occurrence.【Methods】The Hsf family in citrus was identified,and its physicochemical proper-ties,evolutionary development,structural features,cis-acting elements,collinearity,transcriptome,and expression patterns in different tissues of sunburned fruit peel and heat-treated kumquat peel were ana-lyzed.【Results】In this study,14,20,and 16 Hsf genes were identified from the genomes of sweet or-ange,clementine mandarin,and wanbai pomelo,respectively.Physicochemical property analysis re-vealed that the protein lengths and molecular weights were similar among the three citrus varieties,with protein lengths ranging from 271 to 517 amino acids for sweet orange,208 to 505 amino acids for clem-entine mandarin,and 261 to 516 amino acids for wanbai pomelo.The molecular weight ranges were 30.1 to 56.3 ku for sweet orange,24.1 to 56.2 ku for clementine mandarin,and 30.1 to 56.1 ku for wan-bai pomelo.Subcellular localization prediction indicated that all the 50 Hsf genes from the three citrus species were localized in the cell nucleus,consistent with their transcription factor characteristics.Addi-tionally,evolutionary analysis of Hsf genes of sweet orange,clementine mandarin,wanbai pomelo,as well as Arabidopsis,which categorized into three clusters(HSFA,HSFB,and HSFC).The number of members in each cluster followed the pattern of HSFA>HSFB>HSFC,with the fewest members in cluster C,where each species had only one member.Gene structure analysis revealed that members within the same cluster exhibited similar and conserved structural features.Additionally,certain motifs were specific to particular clusters;for instance,motifs 6,8,a

关 键 词：柑橘属 HSF 家族分析 日灼 

分 类 号：S666[农业科学—果树学]