不同LED红蓝光质处理对枳幼苗生长的影响  

Effects of different LED red and blue light quality treatments on the growth of Poncirus trifoliata seedlings

作　　者：周铮荣 赵和国 苏翰英 罗旭钊 孙紫青 马先锋 朱亦赤 ZHOU Zhengrong;ZHAO Heguo;SU Hanying;LUO Xuzhao;SUN Ziqing;MAXianfeng;ZHU Yichi(Engineering Research Center of Horticultural Crop Germplasm Innovation and New Variety Breeding,Ministry of Education/National Center for Citrus Improvement-Changsha/College of Horticulture,Hunan Agricultural University,Changsha 4101258,Hunan,China)

机构地区：[1]园艺作物种质创新与新品种选育教育部工程研究中心·国家柑橘改良中心长沙分中心·湖南农业大学园艺学院,长沙410128

出　　处：《果树学报》2024年第12期2498-2511,共14页Journal of Fruit Science

基　　金：国家重点研发计划(2023YFD1200100);湖南省教育厅科学研究项目(23C0068)。

摘　　要：【目的】探究不同红蓝光LED组合对枳幼苗生长发育的影响。【方法】以无补光为对照(CK),采用红蓝光比例为RB1∶2(红蓝光质比为1∶2,下同)、RB4∶1、RB6∶1、RB8∶1等4个不同比例红蓝光对枳幼苗进行补光处理,并比较其形态和生理生化变化。【结果】经过补光处理210d后,与CK相比,4个补光处理在株高、茎粗、总根长、平均根直径、根尖数和干物质积累量方面均表现显著提升,而根冠比略有降低;叶长和叶宽呈下降趋势,但其长宽比相近;分枝数增加,分枝高度降低;光合参数优化改善,其中RB6∶1的气孔导度和净光合速率达到最高水平。在CK和4个补光处理中,RB6∶1的MDA含量最低,显示出较强的抗性。经过补光处理120d后,4个补光处理的叶片花青苷含量显著高于CK;在补光处理90~210d期间,SPAD值逐渐增大,而色差a^(*)值逐渐减小,并且花青苷含量逐渐降低。【结论】在长沙地区,从11月上旬至次年3月上旬和3月上旬至6月上旬分别采用RB8:1、RB6:1的补光方案,可以实现枳幼苗生长效果的最佳化,并达到茎粗增长量的最大化。【Objective】Citrus is the predominant fruit crop in China,with a plantation area of 2923000 hm^(2) and a yield of 55956000 tons annually as of 2021.The breeding cycle of citrus virus-free container seedlings frequently spans two years,from the initial rootstock sowing to grafting and subsequent nurs-ery cultivation.The protracted breeding cycle leads to a disconnection between citrus seedling supply and demand,resulting in sporadic instances of inadequate availability.Therefore,the citrus seedlings are insufficient to meet the demand of updating and iteration of citrus orchards in China.The extended breeding period also escalates the production costs of citrus virus-free container seedlings,impeding its widespread adoption.Furthermore,limited light exposure is observed during the early spring and au-tumn/winter seasons in most citrus producing regions.The use of greenhouses for seedling production exacerbates the light deprivation experienced by citrus rootstocks.The implementation of supplemental lighting stands out as a viable strategy to improve the light condition,which may be a key initiative to accelerate the citrus rootstock breeding cycle and promote resilient citrus rootstocks.The mechanism by which the mixed red and blue light spectrum modulates the photosynthesis and photomorphogenesis of Poncirus trifoliata seedlings remains unclear.The objective of this study was to determine the optimal supplemental light conditions to improve the growth and development of P.trifoliata seedlings.These results will provide a theoretical insight for accelerating the cycle of citrus virus-free container seed-ling breeding and improving seedling quality.【Methods】P.trifoliata seedlings obtained from Dongk-ou of Hunan province were used as the experimental material.Five light-supplementing treatments were designed,including RB4:1(red to blue light ratio of 4∶1),RB6∶1,RB8∶1,RB1∶2,and no light supplementation(CK).Seedlings with similar growth potential were selected and transplanted in 14 rows,each containing

关 键 词：枳 茎粗 LED补光组合 生长形态 氧化酶活性 

分 类 号：S666.4[农业科学—果树学]