上转换纳米材料在干细胞标记与命运调控中的研究进展  

Advances in Upconversion Nanomaterials for Stem Cell Labeling and Fate Regulation

作　　者：韩骐泽 刘倩 张文乔 胡佩男 张译心 刘瑞敏[1] HAN Qize;LIU Qian;ZHANG Wenqiao;HU Peinan;ZHANG Yixin;LIU Ruimin(Department of Oral and Maxillofacial Surgery,Gansu Provincial Hospital,Lanzhou 730000,China 2.First School of Clinical Medical,Gansu;University of Chinese Medicine,Lanzhou 730000,China;School of Stomatology,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院口腔颌面外科,兰州730000 [2]甘肃中医药大学第一临床医学院,兰州730000 [3]兰州大学口腔医学院,兰州730000

出　　处：《中国生物工程杂志》2024年第11期76-86,共11页China Biotechnology

基　　金：甘肃省青年科技基金(24JRRA610)资助项目。

摘　　要：干细胞是组织工程理想的种子细胞来源,其体内示踪和命运调控对阐述组织修复过程中的作用机制与效果尤为重要。目前已开发多种荧光材料用于生物学领域,其中近红外光激发镧系掺杂上转换荧光纳米颗粒(UCNPs)是一种新型的荧光纳米粒子,能发射紫外光或可见光用于干细胞生物成像或行为调控,具有广泛的应用前景。现对UCNPs进行概述,并重点关注其潜在的示踪与治疗优势。首先简要回顾UCNPs的结构与发光机制,接下来详细介绍增强UCNPs生物学性能的手段。随后,重点阐述UCNPs在干细胞成像、示踪,以及调控成骨向、神经向和软骨向分化的应用。最后,提出UCNPs在干细胞领域应用的局限性和对前景的展望,希望能引起更多研究者对该领域的了解与关注。For tissue engineering,stem cells are a perfect source of seed cells,and their ability to track cells and regulate their fate in vivo is critical to understand the mechanisms and outcomes of tissue repair procedures.Numerous fluorescent materials have been developed for biological applications,among which near-infrared photoexcited lanthanide-doped upconversion nanoparticles(UCNPs)are novel fluorescent nanoparticles capable of emitting ultraviolet or visible light for stem cell bioimaging or fate regulation,which have a wide range of applications.A review of UCNPs is provided,with emphasis on their potential tracer and therapeutic utility.First,the structure and luminescence mechanism of UCNPs are briefly reviewed,followed by a detailed description of the means to enhance the biological properties of UCNPs.The stem cell imaging,tracking,and modulation of osteogenic,neural,and chondrogenic differentiation applications of UCNPs are then described.Finally,the understanding of the constraints and viewpoints of UCNPs in the field of stem cells is presented with the goal of bringing this field to the attention of more researchers.

关 键 词：干细胞 上转换荧光纳米颗粒 成像 分化 生物相容性 

分 类 号：Q819[生物学—生物工程]