微生物细胞合成植物天然产物的耐受工程研究  

Tolerance Engineering of Microbial Cells for the Synthesis of Plant Natural Products

作　　者：王欣欣 王颖 李春 WANG Xinxin;WANG Ying;LI Chun(Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering,Ministry of Industry and Information Technology,Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China;Department of Chemical Engineering,Tsinghua University/Key Laboratory of Industrial Biocatalysis,Ministry of Education,Beijing 100084,China)

机构地区：[1]北京理工大学医药分子科学与制剂工程工信部重点实验室、北京理工大学化学与化工学院生物化工研究所,北京102488 [2]清华大学化学工程系/工业生物催化教育部重点实验室,北京100084

出　　处：《中国生物工程杂志》2024年第11期87-98,共12页China Biotechnology

基　　金：国家重点研发计划(2019YFA0905700、2018YFA0901800)资助项目。

摘　　要：天然产物是一类极具价值的化合物,种类繁多,功能各异,广泛应用于医药、食品及化工等多个领域。随着合成生物学的发展,微生物细胞在合成植物天然产物方面展现出巨大潜力,为药物研发、食品科学及化妆品工业提供了新的生产途径。然而,当前仍面临诸多挑战,植物天然产物多具杀菌、消炎等功效,其在微生物细胞中的过量生产会对细胞产生毒性作用,同时其合成过程中有毒中间体的积累也会加剧细胞毒性。因此,提高微生物对植物天然产物的耐受性尤为重要。近年来,随着对微生物细胞工厂的不断优化,植物天然产物的产量逐步提高,对微生物细胞耐受性的研究也逐渐增多。然而,对于这一领域的系统总结相对较少。因此,对微生物细胞合成植物天然产物耐受方面的研究进行全面综述,重点阐述提升微生物细胞耐受性的策略、当前存在的主要挑战,并对该领域的发展前景进行展望。Natural products are a class of valuable compounds,with a wide range of types and functions,widely used in medicine,food,chemical industry and other fields.With the development of synthetic biology,microbial cells have shown great potential in the synthesis of plant natural products,providing a new production approach for drug research and development,food science and the cosmetics industry.However,there are still many challenges ahead.Plant natural products have many bactericidal,anti-inflammatory and other pharmacological properties.Excessive production in microbial cells leads to toxic effects on the cells.At the same time,the accumulation of toxic intermediates in the synthesis process will also exacerbate cytotoxicity.It is therefore particularly important to improve the tolerance of microorganisms to plant natural products.In recent years,with the continuous regulation of microbial cell factories,the production of plant natural products has gradually increased,along with the research of microbial cell tolerance.However,there is rarely a systematic summary of the research area.Therefore,this paper aims to provide a comprehensive overview of the research on microbial cell tolerance to plant natural products,focusing on the strategies to improve microbial cell tolerance,the current major challenges,and the prospects for the development of this area.

关 键 词：耐受工程 外排泵 适应性进化 代谢调控 植物天然产物 微生物细胞 

分 类 号：Q819[生物学—生物工程]