七叶树Dof转录因子家族鉴定及表达分析  

Identification and expression profiling of Dof transcription factor family in Aesculus chinensis

作　　者：秦晨 解雪华 李路 李俊 米要磊 曹惠妍 孙伟[2] 马伟[1] 万会花 刘秀波[1,3] QIN Chen;XIE Xue-hua;LI Lu;LI Jun;MI Yao-lei;CAO Hui-yan;SUN Wei;MA Wei;WAN Hui-hua;LIU Xiu-bo(School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Jiamusi College,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China;School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]中国中医科学院中药研究所道地药材品质保障与资源持续利用全国重点实验室,北京100700 [3]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [4]广东药科大学药学院,广东广州510006

出　　处：《中国中药杂志》2024年第22期6082-6092,共11页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82304673);中国中医科学院科技创新工程项目(CI2023E002,CI2021A04112)。

摘　　要：七叶树是重要的药用及园艺植物,其干燥成熟种子为著名中药娑罗子,七叶皂苷是其主要活性成分,具有消炎、抗渗出等药理活性,广泛用于腰椎间盘突出、术后水肿、运动损伤等疾病治疗,近年来市场需求持续增加。Dof(DNA binding with one finger)家族是植物界独有的转录因子,在植物的生长发育、抗逆境胁迫以及调控次生代谢等方面发挥重要作用,然而目前关于七叶树Dof基因家族的研究却相对匮乏。该研究从七叶树的基因组中鉴定出36个AcDof基因家族成员,并分析其理化性质、染色体定位、系统发育关系、基因结构、顺式作用元件以及在不同组织部位的表达模式。结果显示:AcDof家族成员的蛋白为81~493个氨基酸,相对分子质量为9270.38~55015.68,等电点为4.84~10.2;亚细胞定位分析表明,34个AcDof蛋白定位于细胞核,其余2个AcDof蛋白定位于叶绿体;系统进化分析将AcDof基因划分为9个亚群,基因结构分析表明所有AcDof基因均拥有C2-C2型单锌指结构域;利用转录组新数据进行基因表达分析发现,AcDof家族成员的表达具有组织特异性,其中AcDof04、AcDof02和AcDof03在种子中具有特异性高表达,表明这些基因可能参与七叶皂苷的合成调控。该研究不仅丰富了人们对七叶树Dof基因家族的认识,更为后续对七叶树中Dof基因的功能和相关调控机制的深入研究提供了重要的基因资源。Aesculus chinensis is an important medicinal and horticultural plant.Its dried mature seeds,known as"Suoluozi",are a well-known traditional Chinese medicine.Aescins are its main active components,possessing multiple pharmacological activities such as anti-inflammatory and anti-exudative effects.They are widely used in the treatment of diseases such as lumbar disc herniation,postoperative edema,and sports injuries,leading to a continuous increase in market demand in recent years.The DNA binding with one finger(Dof)family is a unique transcription factor family found in the plant kingdom.It plays a crucial role in plant growth,development,stress responses,and regulation of secondary metabolism.However,research on the Dof gene family in A.chinensis is relatively scarce.In this study,we identified 36 AcDof genes from the genome of A.chinensis and analyzed their physicochemical properties,chromosomal localization,phylogenetic relationships,gene structures,cis-acting elements,and expression patterns in different tissues.The results showed that AcDof proteins ranged from 81 to 493 amino acids in length,with molecular weights ranging from 9270.38 to 55015.68 and isoelectric points ranging from 4.84 to 10.2.The subcellular localization analysis revealed that 34 AcDof proteins were located in the nucleus,while the remaining two AcDof proteins were located in the chloroplasts.Phylogenetic analysis divided AcDofs into nine subgroups,and gene structure analysis indicated that all AcDof genes possessed a C2-C2 type single zinc finger domain.Gene expression analysis using transcriptome data revealed tissue-specific expression patterns among AcDof family members.Specifically,AcDof04,AcDof02,and AcDof03 exhibited specific expression in seeds,suggesting their potential involvement in the regulation of aescin biosynthesis.This study not only enhances our understanding of the Dof gene family in A.chinensis but also provides important genetic resources for further investigation of the functions and regulatory mechanisms of Dof genes

关 键 词：七叶树 Dof转录因子 基因家族鉴定 生物合成调控 

分 类 号：S567.19[农业科学—中草药栽培]