杜仲Trihelix基因家族全基因组鉴定及表达模式分析  

Genome-wide identification and expression pattern analysis of Eucommia ulmoides Trihelix gene family

作　　者：刘俊 寇杰锋 练从龙 马蕊 冯伟萌 张宝[1,2] 兰金旭 陈随清 LIU Jun;KOU Jie-feng;LIAN Cong-long;MA Rui;FENG Wei-meng;ZHANG Bao;LAN Jin-xu;CHEN Sui-qing(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Key Laboratory of Chinese Medicine Resources and Chemistry,Zhengzhou 450046,China;Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao,Zhengzhou 450046,China;Changjiang Basin Ecology and Environment Monitoring and Scientific Research Center,Changjiang Basin Ecology and Environment Administration,Ministry of Ecology and Environment,Wuhan 430010,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药资源与中药化学重点实验室,河南郑州450046 [3]豫药全产业链研发河南省协同创新中心,河南郑州450046 [4]生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心,湖北武汉430010

出　　处：《中国中药杂志》2024年第22期6093-6106,共14页China Journal of Chinese Materia Medica

基　　金：河南省科技研发计划联合基金项目(222301420074);河南省创新生态支持专项:现代农业产业技术体系建设项目(豫财科[2024]8号)。

摘　　要：Trihelix转录因子在植物光响应、生长发育和逆境胁迫中发挥重要作用,然而杜仲中Trihelix还未见报道。该研究利用生物信息学方法对杜仲Trihelix基因家族进行全面鉴定和表达模式分析,以期为EuGTs基因功能深入研究提供依据。该研究在杜仲中共鉴定出9个Trihelix基因家族成员,编码339~883个氨基酸,等电点位为5.13~9.39,相对分子质量为36992.06~97871.61,亚细胞定位结果显示只有EuGT-2定位在叶绿体上,其余均定位于细胞核中。Teihelix基因家族分为6个亚家族:GT-1、GT-2、SH4、SIP1、GTγ、GTδ,EuGTs分布在SH4、GT-1、GT-2这3个亚家族,分别包含1、6、2个Trihelix蛋白,含有2~17个外显子。EuGTs启动子中含有激素、胁迫、光周期和生长发育等多种顺式作用元件。共线性分析发现杜仲与拟南芥之间有5个共线性基因对,与毛果杨之间共有共线性基因对14个。表达模式分析显示,EuGTs具有组织特异性,EuGT-1、EuGT-2在叶片中表达水平最高,EuGT-4、EuGT-6、EuGT-9在边缘果皮中转录水平最高,EuGT-5、EuGT-8在木质部中表达量最高,随着叶片发育EuGTs呈非同步变化的趋势,EuGTs表达在雄花和雌花中无显著性差异,主要在花的诱导期高量表达。qRT-PCR分析结果表明,大部分EuGTs基因在杜仲叶中表达量最高,EuGT-5在茎中高表达,200 mmol·L^(-1)NaCl处理下,大部分EuGTs基因呈现先升高后降低的表达趋势,显著响应盐胁迫,该研究为进一步探索杜仲EuGTs基因功能和种质创新等提供重要的基因资源。Trihelix transcription factors play important roles in plant light responses,growth and development,and stress responses.However,Trihelix has not yet been reported in Eucommia ulmoides.In this study,bioinformatics methods were used to comprehensively identify and analyze the expression patterns of the Trihelix gene family in E.ulmoides,aiming to provide a basis for further functional studies of EuGTs genes.A total of 9 Trihelix gene family members were identified in E.ulmoides,encoding proteins with 339 to 883 amino acids,with isoelectric points ranging from 5.13 to 9.39 and relative molecular weights between 36992.06 and 97871.61.Subcellular localization results showed that only EuGT-2 was localized in chloroplasts,while the others were located in the nucleus.The Trihelix gene family was categorized into six subfamilies:GT-1,GT-2,SH4,SIP1,GTγ,and GTδ.EuGTs were distributed among three subfamilies:SH4,GT-1,and GT-2,containing 1,6,and 2 Trihelix proteins,respectively,with 2 to 17 exons.The promoters of EuGTs contained various cis-acting elements related to hormones,stress,photoperiod,and growth and development.Collinearity analysis revealed 5 collinear gene pairs between E.ulmoides and Arabidopsis thaliana,and 14 collinear gene pairs between E.ulmoides and Populus.Expression pattern analysis showed that EuGTs exhibited tissue-specific expression:EuGT-1,EuGT-2 had the highest expression levels in leaves,EuGT-4,EuGT-6,EuGT-9 had the highest transcriptional levels in marginal peel,and EuGT-5、EuGT-8 were predominantly expressed in the xylem.As leaves developed,EuGTs showed a trend of asynchronous changes.No significant differences in EuGTs expression were observed between male and female flowers,with high expression levels mainly during the induction stage of flowering.The qRT-PCR analysis indicated that most EuGTs genes were most highly expressed in the leaves of E.ulmoides,while EuGT-5 was highly expressed in the stems.Under 200 mmol·L^(-1)NaCl treatment,most EuGTs genes exhibited an initial increase followed b

关 键 词：杜仲 Trihelix家族 全基因组鉴定 顺式作用元件 表达特征 

分 类 号：S567.19[农业科学—中草药栽培]