基于“药辅互作-成分转化”动态过程探讨甘草炮制附子的科学内涵  

Scientific connotation in processing of Aconiti Lateralis Radix Praeparata with Glycyrrhizae Radix et Rhizoma based on"interactions between excipients and herbal medicine and component transformation"dynamic processing

作　　者：赵一航 王智威 杨璐平 林晓钰 谭心如 徐冉 汪志霞 张留洋 徐安琪 雷海民[1,3] 王鹏龙 黄雪梅[1,2,3] ZHAO Yi-hang;WANG Zhi-wei;YANG Lu-ping;LIN Xiao-yu;TAN Xin-ru;XU Ran;WANG Zhi-xia;ZHANG Liu-yang;XU An-qi;LEI Hai-min;WANG Peng-long;HUANG Xue-mei(School of Chinese Materia Medica,Bejing University of Chinese Medicine,Beijing 102488,China;Traditional Chinese Medicine Processing Technology Inheritance Base of National Administration of Traditional Chinese Medicine,Beijing 102488,China;Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]国家中医药管理局中药炮制技术传承基地,北京102488 [3]中药品质评价北京重点实验室,北京102488

出　　处：《中国中药杂志》2024年第22期6129-6137,共9页China Journal of Chinese Materia Medica

基　　金：北京市自然科学基金项目(7242221);国家自然科学基金项目(82274072);中央高校基金科研业务项目(2024-JYB-XJSJJ004,2023-JYB-JBZD-049,2022-XJ-KYQD-008,2023-JYB-KYPT-17);国家级高层次青年人才支持计划项目;2024年北京中医药大学大学生创新创业训练计划创新训练项目(202410026004)。

摘　　要：中药炮制是中医药核心理论之一,蕴含深厚的哲学、中华文化和自然科学智慧,其中“药辅共制”工艺因其独特性而备受关注。该研究通过酸性染料比色法、UPLC-Q-TOF-MS/MS、密度泛函理论、分子动力学模拟等多种技术探讨辅料甘草的引入对附子炮制时成分转化动态过程的影响。研究发现,炮制过程中附子多种生物碱成分与辅料甘草中的甘草酸之间存在着不同的弱相互作用,影响生物碱类成分(乌头碱、次乌头碱等双酯型生物碱)的转化和含量变化。该研究基于“药辅互作-成分转化”动态过程角度,揭示了甘草炮制附子的内在机制,为阐明中药辅料炮制科学内涵提供参考。The processing of traditional Chinese medicine(TCM)is a core theory within TCM,embodying deep philosophical,cultural,and natural scientific wisdom.Among the various techniques,the"synergistic processing of medicinal materials and excipients"has garnered significant attention due to its uniqueness.This study explored the impact of the adjuvant Glycyrrhizae Radix et Rhizoma on the dynamic process of component transformation during the processing of Aconiti Lateralis Radix Praeparata using techniques such as acidic dye colorimetry,UPLC-Q-TOF-MS/MS,density functional theory(DFT),and molecular dynamics simulations(MDS).The research revealed that during processing,various alkaloid components in Aconiti Lateralis Radix Praeparata exhibited different weak interactions with glycyrrhizic acid in Glycyrrhizae Radix et Rhizoma,affecting the transformation and content changes of alkaloid components such as aconitine,hypaconitine,and other diester-type alkaloids.This study,based on the dynamic process of"interactions between excipients and herbal medicine and component transformation",elucidated the intrinsic mechanism of processing of Aconiti Lateralis Radix Praeparata with Glycyrrhizae Radix et Rhizoma and provided a reference for understanding the scientific principles underlying the excipient processing of TCM.

关 键 词：甘草 附子 甘草酸 生物碱 相互作用 

分 类 号：R283[医药卫生—中药学]