露水草甾酮类化学成分及其体外抗HSV-1活性研究  

Isolation and identification of steroids from Cyanotis arachnoidea and their anti-HSV-1 activities in vitro

作　　者：苏芳谊 李波 肖斌 万洪 刘丁 张春磊 SU Fang-yi;LI Bo;XIAO Bin;WAN Hong;LIU Ding;ZHANG Chun-lei(School of Life Science,Zhengzhou Normal University,Zhengzhou 450044,China;Chengdu Push Bio-Technology Co.,Ltd.,Chengdu 610045,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]郑州师范学院生命科学学院,河南郑州450044 [2]成都普思生物科技股份有限公司,四川成都610045 [3]中国药科大学中药学院,江苏南京211198

出　　处：《中国中药杂志》2024年第22期6165-6172,共8页China Journal of Chinese Materia Medica

基　　金：河南省科技攻关项目(242102310444);河南省高等学校重点科研项目(23A360027,23A180024);郑州师范学院大学生创新训练重点项目(DCZ2023013)。

摘　　要：研究露水草提取物中的甾酮类化学成分及其体外抗单纯疱疹病毒(HSV-1)活性。采用硅胶、ODS、Sephadex LH-20等柱色谱和半制备高效液相色谱等方法对露水草75%乙醇提取物中乙酸乙酯部位进行分离纯化,并根据波谱数据进行结构鉴定。该研究从露水草中分离鉴定了10个甾酮类化合物,分别为makisterone A 3-acetate(1)、makisterone A 2-acetate(2)、makisterone A(3)、ajugasterone C 2-acetate(4)、ajugasterone C(5)、20-hydroxyecdysone 2-acetate(6)、20-hydroxyecdysone 3-acetate(7)、podecdysone C(8)、rubrosterone(9)、poststerone(10)。化合物1和2为2个新的甾酮类化合物。采用CPE抑制法检测化合物1~10的抗HSV-1活性,实验采用Vero细胞,以acyclovir为阳性对照,结果表明化合物9和10具有一定的抗HSV-1活性,其EC50分别为83.11、103.62μg·mL^(-1)。The steroid constituents in the 75%ethanol extract of Cyanotis arachnoidea and their anti-HSV-1 activities in vitro were investigated in this study.The ethyl acetate fraction of the extract was isolated and purified using silica gel,ODS,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Structural identification was conducted based on spectroscopic data.Ten steroid compounds were isolated and identified,namely makisterone A 3-acetate(1),makisterone A 2-acetate(2),makisterone A(3),ajugasterone C 2-acetate(4),ajugasterone C(5),20-hydroxyecdysone 2-acetate(6),20-hydroxyecdysone 3-acetate(7),podecdysone C(8),rubrosterone(9),and poststerone(10).Compounds 1 and 2 are new steroid compounds.The anti-HSV-1 activities of compounds 1-10 were evaluated using the CPE inhibition method.Vero cells were used in the experiment,with acyclovir as the positive control.The results showed that compounds 9 and 10 showed anti-HSV-1 activity,with EC_(50)values of 83.11 and 103.62μg·mL^(-1),respectively.

关 键 词：露水草 化学成分 结构鉴定 HSV-1 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]