肠道菌群与斑秃的因果关系研究:一项双向双样本孟德尔随机化研究  

Causal relationship between gut microbiota and alopecia areata:a bidirectional two-sample Mendelian randomization study

作　　者：吕兴民 徐茂婷 陈贵全 LYU Xingmin;XU Maoting;CHEN Guiquan(Department of Acupuncture,Massage and Rehabilitation,The Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000,China;College of Integration of Traditional Chinese and Western Medicine To Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医院针灸推拿康复科,四川沪州646000 [2]西南医科大学中西医结合学院,四川泸州646000

出　　处：《中国麻风皮肤病杂志》2025年第1期15-22,共8页China Journal of Leprosy and Skin Diseases

基　　金：第五批全国中医临床优秀人才研修项目(编号:国中医药人教函〔2022〕1号);西南医科大学校级科研项目(编号:2021ZKZD020)。

摘　　要：目的:明确肠道菌群与斑秃之间的因果关系,为早期斑秃预防和疾病改善提供参考。方法:选取与肠道菌群相关的显著单核苷酸多态性(SNP)作为工具变量,采用逆方差加权法(IVW)评估因果效应。通过Cochran's Q检验评估遗传工具变量的异质性,MR-Egger截距检验和MR-PRESSO检验评估多效性,“留一法”评估SNPs对斑秃因果关系的影响敏感性。结果:逆方差加权法显示,毛螺菌科3-1-46FAA群、戴阿利斯特杆菌属、副拟杆菌属、瘤胃球菌属lactaris群、戴阿利斯特杆菌属invisus群、PWY.6731途径相关菌群、拟杆菌属faecis群与斑秃呈负相关;PWY0.1415途径相关菌群、拟杆菌门、拟杆菌目、拟杆菌纲、拟杆菌属caccae群、PWY.5686途径相关菌群与斑秃呈正相关;反向孟德尔随机化结果显示,斑秃与拟杆菌目、拟杆菌纲、拟杆菌门成正相关;敏感性分析:MR-Egger截距检验和MR-PRESSO检验表明不存在水平多效性,异质性检验显示两者关系无异质性,“留一法”分析结果稳健。结论:肠道微生物群中拟杆菌相关细菌性状与斑秃的发病有关。Objective:To determin the relationship between gut microbiota and alopecia areata,and to provide reference for early prevention and disease improvement of alopecia areata.Methods:The study selected significant SNPs associated with gut microbiota as instrumental variables and used the inverse variance weighted(IVW)method as the primary analysis method for assessing causal effects.Heterogeneity of the genetic instrumental variables was evaluated with Cochran's Q test,and pleiotropy was assessed with the MR-Egger intercept test and the MR-PRESSO test.Sensitivity of the causal relationship between SNPs and alopecia areata was evaluated using the leave-one-out approach.Results:The inverse variance weighted method showed that the Lachnospiraceae family group 3-1-46FAA,Dialister genus,Paraprevotella genus,Ruminococcus genus lactaris group,Dialister invisus group,and the PWY.6731 pathway microbiota were negatively correlated with alopecia areata;the PWY0.1415 pathway microbiota,Bacteroidetes phylum,Bacteroidales order,Bacteroidia class,Bacteroides genus caccae group,and PWY.5686 pathway microbiota were positively correlated with alopecia areata;Mendelian randomization results showed a positive correlation between alopecia areata and the Bacteroidales order,Bacteroidia class,and Bacteroidetes phylum;Sensitivity analysis:The MR-Egger intercept test and MR-PRESSO test indicated no horizontal pleiotropy,and the heterogeneity test showed no heterogeneity in their relationship,with the leave-one-out analysis yielding robust results.Conclusion:The gut microbiota of Bacteroides were associated with alopecia areata.

关 键 词：斑秃 肠道菌群 孟德尔随机化 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]