大鼠血浆中米托坦的测定及其药动学研究  

Determination and pharmacokinetic study of Mitotane in rats plasma

作　　者：于娜 张志荣[1] YU Na;ZHANG Zhirong(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2024年第6期689-691,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的测定大鼠血浆中米托坦的含量,并研究米托坦在大鼠体内的药动学。方法用乙睛沉淀预处理血浆样品,采用HPLC法检测米托坦在大鼠血浆中的含量,运用DAS3.0软件分析药动学数据。结果米托坦回归方程为:Y=1.296×10^(5)X+5.823×10^(3)(r^(2)=0.9999),线性范围0.025~8.008μg·mL^(-1);绝对回收率、方法回收率分别为97.64%~99.98%、99.93%~102.09%,精密度RSD均≤1.01%(n=3)。米托坦静注与灌胃给药的C_(max)有显著性差异。结论所用方法符合生物样品分析的基本要求,适合用于测定米托坦在大鼠体内的含量。采用静注给药可显著提高米托坦的生物体内达峰浓度。OBJECTIVE To determinate the Mitotane in rats plasma and to study perform pharmacokinetics of Mitotane in rats.METHODS The rats plasma samples underwent pretreatment using the organic solvent precipitation method with acetonitrile as the precipitation agent.The concentration of Mitotane was determined using HPLC,and the resulting pharmacokinetic data were analyzed using DAS 3.O sofware.RESULTS The regression equation of Mitotane was Y=1.296×10^(5)X+5.823×10^(3)(r^(2)=0.9999),with the linear range of 0.025-8.008μg·mL^(-1).The absolute and method recoveries were between 97.64%and 99.98%,and between 99.93%and 102.09%,respectively.The precision RSD was≤1.01%(n=3).The C_(max) of Mitotane iv differed significantly from that of ig.CONCLUSION The HPLC analysis of Mitotane plasma concentration meets the basic requirements for biological samples,and is suitable for the determination of mitotane in rats.Intravenous administration can significantly increase the peak concentration of Mitotane in the organ.

关 键 词：米托坦 高效液相色谱法 药动学 体内含量测定 有机溶剂沉淀法 肾上腺皮质癌 杀虫剂 生物样品预处理 

分 类 号：R917[医药卫生—药物分析学]