UPLC-MS/MS鉴定北葶苈子的化学成分  

Analysis of chemical components of Lepidium apetalum by UPLC-MS/MS

作　　者：周德勇 宋京美 王夏 龚韬 贾利影 李蜀平 ZHOU Deyong;SONG Jingmei;WANG Xia;GONG Tao;JIA Liying;LI Shuping(Beijing Research Institute of Chinese Medicine,Beijing,100010 P.R.China;Beijing Hospital of TCM,Capital Medical University,Beijing,100010 P.R.China)

机构地区：[1]北京市中医药研究所,北京1000101 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《华西药学杂志》2024年第6期697-704,共8页West China Journal of Pharmaceutical Sciences

基　　金：北京市中医药研究所市财政专项(YJS-2023-18)。

摘　　要：目的采用超高效液相色谱-质谱联用法快速鉴定北葶苈子药材的化学成分。方法采用Waters UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm),柱温40℃,以甲醇-0.1%甲酸为流动相,梯度洗脱,在正负离子模式下采集高分辨质谱数据。结果通过保留时间、高分辨精确分子量、MS/MS碎片等信息,结合对照品数据及文献对比分析,共鉴定了95个化合物,包括黄酮类24个、脂肪酸类19个、苯丙素类14个、硫苷及异硫氰酸类11个、尿苷5个、其他类22个。结论快速地分析北葶苈子中化学成分,总结了化合物的裂解规律,为其成分分析、质量控制、临床应用等提供参考。OBJECTIVE To rapidly identify the chemical constituents in Lepidium apetalum Willd.by UPLC-Q-Exactive Orbitrap MS.METHODS Waters UPLC HSS T3 column(100 mm×2.1 mm,1.8μm)was used,with column temperature of 40℃.Gradient elution was performed with methanol-0.1%formic acid as the mobile phase,and the high-resolution mass spectrometry data were collected in positive and negative ion modes.RESULTS Based on the retention time,high-resolution accurate molecular weight,MS/MS fragmentation and other information,combined with reference substance data,95 compounds were identified from L.apetalum,including 24 flavonoids,19 fatty acids,14 phenylpropanoids,11 glucosinolates and isothiocyanates,5 uridines,and 22 others.CONCLUSION The method can quickly and relatively resolved the chemical constituents in L.apetalum,summarize the cleavage law of the compounds.This study provides reference and scientific basis for the component analysis,quality control and clinical application of L.apetalum.

关 键 词：北葶苈子 超高效液相色谱串联四极杆静电场轨道阱质谱联用 黄酮类 脂肪酸类 硫苷、异硫氰酸类 苯丙素类 裂解规律 

分 类 号：R917[医药卫生—药物分析学]