UPLC多波长法测定补肾益脑胶囊中的10种成分  

Determination of 10 components in Bushen Yinao capsules by UPLC with multi-wavelength

作　　者：朱泽兵 郭娅 牟腊梅 张德伟 向琴 何旭峰 ZHU Zebing;GUO Ya;MOU Lamei;ZHANG Dewei;XIANG Qing;HE Xufeng(Chongqing Wanzhou Food and Drug Inspection Institute,Chongqing,404100 P.R.China;Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in the Three Gorges Reservoir Area,Chongqing Three Gorges Medical College,Chongqing,404100 P.R.China)

机构地区：[1]重庆市万州食品药品检验所,重庆404100 [2]重庆三峡医药高等专科学校三峡库区道地药材开发利用重庆市重点实验室,重庆404100

出　　处：《华西药学杂志》2024年第6期713-716,共4页West China Journal of Pharmaceutical Sciences

基　　金：重庆市科学技术局与重庆市药品监督管理局2022年度药品科研项目(渝药监[2022]27号);三峡库区道地药材开发利用重庆市重点实验室项目(Sys20210028)。

摘　　要：目的采用超高效液相色谱法(UPLC)同时测定补肾益脑胶囊中的10种成分。方法采用Acquity UPLC BEH-C18色谱柱(100 mm×2.1 mm,1.7μm),流动相为乙腈-0.1%磷酸水溶液,进行梯度洗脱,流速0.3 mL·min^(-1),柱温30℃,进样量1μL,检测波长分别为203 nm(人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、哈巴俄苷)、250 nm(补骨脂素、异补骨脂素、五味子醇甲、五味子酯甲)、320 nm(阿魏酸、斯皮诺素)。结果阿魏酸、斯皮诺素、补骨脂素、异补骨脂素、人参皂苷Rg1、人参皂苷Re、哈巴俄苷、人参皂苷Rb1、五味子醇甲、五味子酯甲浓度分别在13.15~210.40、2.58~41.35、1.16~18.58、1.64~26.22、6.03~96.41、7.21~115.30、1.28~20.48、24.50~392.00、1.16~18.64、2.42~38.68μg·mL^(-1)时与峰面积呈良好的线性关系,其r均大于0.9998,平均回收率分别为92.8%、92.0%、97.3%、97.8%、91.0%、102.3%、95.3%、99.3%、101.4%、96.7%,RSD分别为1.30%、1.78%、2.65%、1.95%、1.86%、3.12%、1.09%、1.35%、1.60%、1.85%。结论所用方法简便可靠、重复性好,可用于补肾益脑胶囊中10种成分的含量测定。OBJECTIVE To determine the 10 components in Bushen Yinao capsules(BYC)by UPLC.METHODS The chromatographic column was Acquity UPLC BEH-C_(18)(100 mm×2.1 mm,1.7μm).The mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution.Gradient elution was performed at the flow rate of 0.3 mL·min^(-1),the column temperature was 30℃,and the sample volume was 1μL.The detection wavelengths were 203 nm,250 nm,and 320 nm,respectively.RESULTS The mass concentrations of ferulic acid,spinosin,psoralatin,isopsoralatin,ginsenoside Rg1,ginsenoside Re,harbacoside,ginsenoside Rb1,schisandrin and schisantherin A were 13.15-210.40,2.58-41.35,1.16-18.58,1.64-26.22,6.03-96.41,7.21-115.30,1.28-20.48,24.50-392.00,1.16-18.64,1.16-18.64,2.42-38.68μg·mL^(-1).There was a good linear relationship with the peak area,and the r were greater than 0.9998.The average recoveries were 92.8%,92.0%,97.3%,97.8%,91.0%,102.3%,95.3%,99.3%,101.4%,96.7%,and the RSD values were 1.30%,1.78%,2.65%,1.95%,1.86%,3.12%,1.09%,1.35%,1.60%,1.85%,respectively.CONCLUSION The method is simple,reliable and reproducible,and can be used for the determination of 10 components in BYC.

关 键 词：补肾益脑胶囊 活性成分 多波长 同时测定 超高效液相色谱法 质量标准 含量测定 

分 类 号：R917[医药卫生—药物分析学]