悬浊液转移方式对不同CO_(2)浓度下麦田土壤胞外酶活性测定的影响  

Effect of Transfer Methods on Measurement of Extracellular Enzyme Activities in Winter Wheat Field under Different CO_(2) Concentration

作　　者：白家韶 韩雪[1] 张馨月 鲍秀蕊 许吟隆[1] BAI Jia-shao;HAN Xue;ZHANG Xin-yue;BAO Xiu-rui;XU Yin-long(Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agriculture Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《中国农业气象》2024年第12期1405-1416,共12页Chinese Journal of Agrometeorology

基　　金：国家重点研发计划(2019YFA0607403);国家自然科学基金项目(31901174);中国农业科学院科技创新工程农业绿色低碳科学中心专项项目(CAAS-CSGLCA-202301);中央级公益性科研院所基本科研业务费(BSRF202202)。

摘　　要：因胞外酶活性测定过程中土壤悬浊液转移方式(搅拌或静置)不统一,其测定结果是否一致有待试验验证。为探讨悬浊液转移方式对不同环境条件下土壤胞外酶活性测定结果的影响,本研究依托北京昌平农田试验站开放式CO_(2)富集平台,在对照(420±15μmol·mol-1)和CO_(2)浓度升高(550±15μmol·mol-1)条件下采集冬小麦不同生育期的表层土壤,比较评价搅拌和静置两种转移方式对7种胞外酶活性的影响和差异。结果表明:搅拌转移方式测定的胞外酶活性高于静置转移,可能与搅拌转移方式包含更多的络合吸附胞外酶的土壤颗粒有关。不同类型胞外酶活性受转移方式的影响程度不同。其中,碳(C)、磷(P)获取酶活性受转移方式影响较大,搅拌转移方式较静置转移方式的测定结果显著提高了191.45%~774.09%,氮(N)获取酶活性受影响相对较小,搅拌转移方式较静置转移方式的结果仅提高了6.66%~30.59%。转移方式还导致胞外酶活性对CO_(2)浓度升高的响应不同,搅拌转移下酶活性基本不响应CO_(2)浓度升高,静置转移下大部分酶活性随CO_(2)浓度升高显著降低。胞外酶化学计量比和矢量特征均表明搅拌转移方式下微生物资源限制不受CO_(2)浓度升高的影响,静置转移方式下CO_(2)浓度升高缓解了微生物C限制程度,并增加了其N限制程度。本研究证实土壤悬浊液转移至微孔板的方式不仅影响酶活性、酶化学计量比和矢量特征,还影响上述指标对CO_(2)浓度升高的响应,进而使CO_(2)浓度升高下表征微生物的资源限制结果不同。本研究呼吁统一转移方式,减少研究结果的不确定性。The soil slurry transfer methods(e.g.transferring soil slurry when it was continuously agitating or reached standing state)for extracellular enzyme activity(EEA)measurement are still different.Whether the result of EEAs is consistent between the two transfer methods remains unclear and needs to be tested.This research aimed to estimate the impact of transfer methods on EEA under different environmental conditions.Surface soil was collected in different growth stages of winter wheat.The winter wheat field was located in a free air carbon dioxide enrichment(FACE)platform in Changping district of Beijing.The FACE platform included two CO_(2) concentrations,which were ambient CO_(2) concentration(420±15μmol·mol-1)and elevated CO_(2) concentration(550±15μmol·mol-1),respectively.Seven EEAs were chosen to study the influence of different transfer methods under different CO_(2) concentrations.The results showed that the EEAs were significantly higher under agitating state than standing state.It might be attributed to the fact that soil slurry under agitating state contained more soil particles,which were easily to absorb extracellular enzymes.The extent of influence of transfer methods on EEA varied among different enzymes.To be specific,the carbon(C)and phosphorus(P)acquiring enzyme activities were strongly influenced by transfer methods,showing a significant increase of 191.45%to 774.09%under agitating state compared to standing state,while the nitrogen(N)acquiring enzyme activity only showed an increase of 6.66%to 30.59%under agitating state compared to standing state.The transfer methods also led to different responses of enzyme activities to elevated CO_(2).Most enzyme activities showed no response to elevated CO_(2) under the agitating state.By contrast,more enzyme activities decreased significantly in response to elevated CO_(2) under the standing state.Furthermore,the calculated extracellular enzymatic stoichiometry and vector characteristic both indicated that microbial resource limitation was unchanged by

关 键 词：胞外酶活性 转移方式 华北冬小麦 FACE平台 CO_(2)浓度升高 

分 类 号：S512.1[农业科学—作物学] S154.2