耗散粒子动力学模拟细胞在硬基底上的粘附铺展  

Dissipative particle dynamics simulation of cell adhesion on rigid substrate

作　　者：冯才懿 王鑫[1,2] FENG Caiyi;WANG Xin(Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control,Tianjin University of Technology,Tianjin 300384,China;National Demonstration Center for Experimental Mechanical and Electrical Engineering Education,Tianjin University of Technology,Tianjin 300384,China)

机构地区：[1]天津理工大学天津市先进机电系统设计与智能控制重点实验室,天津300384 [2]天津理工大学机电工程国家级实验教学示范中心,天津300384

出　　处：《天津理工大学学报》2024年第6期117-122,共6页Journal of Tianjin University of Technology

基　　金：天津市自然科学基金(21JCYBJC01210);天津市透明质酸应用研究企业重点实验室开放基金项目(KTRDHA-Y201904)。

摘　　要：生物体内或体外培养的细胞基本都处于粘附状态,在生物体内,组织和器官会受到由细胞与胞外基质、细胞与细胞等相互作用产生的力;在体外,细胞在基底上粘附、迁移和铺展,细胞会受到基底对其力的作用。胞外力学微环境的改变会导致细胞骨架重构,细胞形态会发生改变,因此定量地分析细胞由悬浮到粘附的过程对于了解粘附细胞的生物学行为具有一定意义。文中借助耗散粒子动力学(dissipative particle dynamics,DPD)的研究方法,通过矩阵实验室(matrix laboratory,MATLAB)建立了一个可以表征大多数真核细胞的细胞模型,通过大规模原子/分子并行器(large-scale atomic/molecular massive parallel simulator,LAMMPS)进行模拟,模拟了细胞由悬浮状态到粘附状态的过程及对于不同粘附力的力学响应,细胞在不同的粘附力作用下发生了机械重塑表现出不同的形态,可以为生物力学和机械生物学的研究提供一定的参考。Cells seeded in an organism or cultured in vitro are essentially in a state of adhesion.In living organisms,all tissues and organs are subject to forces generated by cell-extracellular matrix,cell-cell,and other interactions.In vitro experiments,in the process of adhesion,migration,and spreading,cells are subject to the forces exerted by substrate.Changes in the microenvironment of extracellular mechanics lead to cytoskeletal remodeling and consequent changes in cell morphology.Therefore,quantitative analysis of the dynamic process of cell suspension to adhesion is of interest to understand the biological behavior of adherent cells.In this paper,a cell model representing most eukaryotic cells was established by matrix laboratory(MATLAB),and simulated by large-scale atomic/molecular massive parallel simulator(LAMMPS),a molecular dynamics software,based on dissipative particle dynamics.This paper is to simulate the process from suspension to adhesion and the mechanical response of cells to different adhesion forces.The cells undergo mechanical remodeling under the action of various adhesion forces exhibiting alternative morphologies,which provides significant reference for the study of cellular biomechanics and mechanobiology.

关 键 词：耗散粒子动力学 粘附细胞 大规模原子/分子并行器 力学响应 粘附力 

分 类 号：Q66[生物学—生物物理学]