自然低温下二十二个蝴蝶兰品种的抗寒性评价  

Evaluation of Cold Resistance in 22 Phalaenopsis Varieties Under Natural Low Temperature

作　　者：谢振兴[1] 刘国敏 陆祖正[1] 黄歆怡 覃茜[1] 罗清[1] XIE Zhenxing;LIU Guomin;LU Zuzheng;HUANG Xinyi;QIN Qian;LUO Qing(Guangxi Subtropical Crops Research Institute/Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530001;Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)

机构地区：[1]广西壮族自治区亚热带作物研究所,农业农村部亚热带果品蔬菜质量安全控制重点实验室,广西南宁530001 [2]广西壮族自治区农业科学院经济作物研究所,广西南宁530007

出　　处：《北方园艺》2024年第23期58-65,共8页Northern Horticulture

基　　金：广西农业科学院基本科研业务专项资助项目(桂农科2022YM15,桂农科2021YT152);广西自然科学基金资助项目(2022GXNSFBA035455)。

摘　　要：以引进的22个蝴蝶兰品种为试材,采用主成分分析法、隶属函数分析法和聚类分析法,研究了不同蝴蝶兰在自然低温胁迫后相对电导率(REC)、丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、可溶性蛋白质(SP)、可溶性糖(SS)、脯氨酸(Pro)、磷酸烯醇式丙酮酸羧化酶(PEPC)等9个抗寒性生理指标的变化,以期为蝴蝶兰抗寒种质挖掘及抗寒性评价提供参考依据。结果表明:22个蝴蝶兰品种中丙二醛、过氧化氢酶、超氧化物歧化酶、过氧化物酶、可溶性蛋白质、可溶性糖、脯氨酸、磷酸烯醇式丙酮酸羧化酶等8个指标差异显著。主成分分析发现,前4个主成分的累积贡献率为73.706%,解释了大部分抗寒性指标的信息。以综合抗寒能力D值进行聚类分析可将22个蝴蝶兰品种划分成5类,其中抗寒性最强的品种是‘版纳’‘罗氏’‘西蕾丽’,抗寒性最差的是‘蓝星’‘紫钻’‘香妃’。Taking 22 introduced Phalaenopsis varieties as the test materials,principal component analysis,subordinate function analysis and cluster analysis were used to study the changes of nine physiological indicators of cold resistance in different Phalaenopsis after natural low temperature stress,including relative electric conductivity (REC),malondialdehyde (MDA),catalase (CAT),superoxide dismutase (SOD),peroxidase (POD),soluble protein (SP),soluble sugar (SS),proline (Pro) and phosphoenolpyruvate carboxylase (PEPC),in order to provide reference for the mining of cold-resistant germplasm resources and evaluation of cold resistance in Phalaenopsis.The results showed that eight indicators of MDA,CAT,SOD,POD,SP,SS,Pro and PEPC showed significant differences among the 22 Phalaenopsis varieties.Principal component analysis found that the cumulative contribution rate of the first four principal components was 73.706%,explaining most of the information of cold resistance indicators.Cluster analysis based on the comprehensive cold resistance ability D value could divide the 22 Phalaenopsis varieties into five categories.Among them,the strongest cold-resistant varieties were Phalaenopsis mannii,lobbi,and schilleriana,while the weakest cold-resistant varieties were Phalaenopsis ‘lanxing’‘zizuan’,and ‘xiangfei’.

关 键 词：蝴蝶兰 抗寒性 主成分分析 隶属函数分析 聚类分析 

分 类 号：S682.31[农业科学—观赏园艺]