抑制鸢尾果枯病生防细菌的筛选及条件优化  

Screening and Optimization of Conditions for the Inhibition of Iris sanguine Fruit Blight Biocontrol Bacteria

作　　者：朱桐 王宇曦 逯昕明 刘雪峰[1] 王斌 刁桂萍[1] ZHU Tong;WANG Yuxi;LU Xinming;LIU Xuefeng;WANG Bin;DIAO Guiping(Forestry School,Northeast Forestry University,Harbin,Heilongjiang 150040;Institute of Natural Resources and Ecology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150040;Ordos Forestry and Grassland Development Center,Ordos,Inner Mongolia 017000)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040 [3]鄂尔多斯市林业和草原事业发展中心,内蒙古鄂尔多斯017000

出　　处：《北方园艺》2024年第23期66-72,共7页Northern Horticulture

基　　金：中央高校基本科研业务费专项资金资助项目(2572022DS07)。

摘　　要：以鸢尾果枯病(Iris sanguine fruit blight)病原菌交链格孢菌(Alternaria alternata)和几种植物内生细菌为试材,采用平板对峙法与单因素试验法,研究了不同内生细菌对A.alternata的抑制作用以及所筛选出的高效生防细菌在不同培养条件下的抑菌能力与稳定性,以期为鸢尾果枯病及相关病害的防治和生防菌剂的开发提供参考依据。结果表明:枯草芽胞杆菌(Bacillus subtilis)和贝莱斯芽孢杆菌(B.velezensis)对A.alternata具有较强的抑制力;二者最佳培养基和培养条件为在pH=5的LB液体培养基中装入5%的种子液,置于30℃、180 r·min^(-1)条件下培养16 h;B.subtilis的酸碱、热、紫外线照射和光照稳定性较好,B.velezensis除热稳定性较高外,其他处理下抑菌效果均下降。Taking the plate standoff method and one-way test with Iris sanguine fruit blight pathogen A.alternata and several plant endophytic bacteria as the test materials,the inhibitory effects of different endophytic bacteria on A.alternataas well as the inhibitory ability and stability of the screened efficient biocontrol bacteria under different culture conditions were studied,in order to provide reference for the prevention and control of Iris sanguine fruit blight and related diseases and the development of biocontrol agents.The results showed that Bacillus subtilis and B.velezensis had substantial inhibitory effects on A.alternata.For both of them,the following cultivation conditions and medium were ideal with adding 5%seed solution to LB liquid medium at pH=5.It was incubated for 16 hours at 30℃and 180 r·min^(-1).B.subtilis had better acid-base,heat,UV irradiation,and light stability.In addition to the higher heat stability of B.velezensis,the antibacterial effect of other treatments decreased.

关 键 词：链格孢 芽孢杆菌 生防菌 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S476.7[农业科学—植物保护]