乙醇--硫酸铵双水相体系提取独一味环烯醚萜苷及活性验证  

EXTRACTION OF IRIDOID GLYCOSIDES FROM ETHANOL-AMMONIUM SULFATE AQUEOUS TWOPHASE SYSTEM AND VERIFICATION OF ITS ACTIVITY

作　　者：乔茂旺 张国燕[1,2] 陈海娟 尚军[1,2] QIAO Mao-wang(Qinghai normal University,key Laboratory of Medicinal Animal and Plant Resources in Qinghai-Xizang Plateau,Xining Qinghai 81008,China)

机构地区：[1]青海师范大学/青海省青藏高原药用动植物资源重点实验室,青海西宁810008 [2]高原科学与可持续发展研究院,青海西宁810000

出　　处：《青海草业》2024年第4期22-30,共9页Qinghai Prataculture

基　　金：青海省科技厅国际合作项目(2021-HZ-805);青海省“昆仑英才·高端创新创业人才”(2020)。

摘　　要：以不同质量分数的乙醇-硫酸铵双水相体系提取独一味环烯醚萜苷类化合物,以8-O-乙酰山栀苷甲酯为标准品测定其含量;并通过细胞实验验证其抗炎效果。结果表明:当乙醇含量为28%,硫酸铵含量为22%,环烯醚萜苷得率可达327.14 mg/g,且经体外细胞抗炎实验,所得环烯醚萜苷活性部位在不同剂量下均可显著降低RAW 264.7巨噬细胞炎症水平(P<0.05)。双水相萃取可有效富集独一味中环烯醚萜苷类成分,效果显著。Iridoid terpenes were extracted from ethanol-ammonium sulfate aqueous two-phase system with different mass fractions,and the content of iridoid terpenes was determined by using 8-O-acetylgeniposide methyl ester as standard.And its anti-inflammatory effect was verified by cell experiment.Results when the content of ethanol was 28%and the content of ammonium sulfate was 22%,the yield of iridoid glycosides could reach327.14mg/g.And the results verified by in vitro cell anti-inflammatory experiments showed that the obtained active site of iridoid glycosides could significantly reduce the inflammation level of RAW264.7 macrophages at different doses(P<0.05).Aqueous two-phase extraction can can effectively enrich the iridoid glycosides in Lamiophlomis rotata,and the effect is significant.

关 键 词：独一味 乙醇-硫酸铵双水相 环烯醚萜苷 抗炎 

分 类 号：S567.239[农业科学—中草药栽培] R285[农业科学—作物学]