补阳还五汤对MCAO大鼠rt-PA溶栓后神经功能及血清炎症因子的影响  

Effects of Buyang Huanwu decoction on nerve function and serum inflammatory factors after rt-PA thrombolysis in MCAO rats

作　　者：邢锡熙 胡靖 吴勉华 李文磊[3] 梅枝忠 丁煜 王庆敏 XING Xixi;HU jing;WU Mianhua;LI Wenlei;MEI Zhizhong;DING Yu;WANG Qingmin(Department of Encephalology,Nanjing Jiangning Hospital of Chinese Medicine,Nanjing 211000,CHINA)

机构地区：[1]南京市江宁中医院(中国药科大学附属江宁中医院)脑病科,江苏211000 [2]南京中医药大学第一临床医学院 [3]江苏省中医院脑病中心

出　　处：《江苏医药》2024年第11期1081-1085,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金项目(81973794);江苏省中医药科技发展计划项目(MS2021042);南京中医药大学自然科学基金项目(XZR2020067)。

摘　　要：目的 探讨补阳还五汤对大脑中动脉栓塞(MCAO)大鼠重组组织型纤溶酶原激活剂(rt-PA)溶栓后神经功能及血清炎症因子的影响。方法 24只SPF级雄性大鼠随机均分为四组,假手术组(A组)、模型组(B组)、rt-PA组(C组)和补阳还五汤+rt-PA组(D组)。B、C、D组建立大鼠MCAO模型。造模成功后,C、D组大鼠经尾静脉注射rt-PA 10mg/kg溶栓治疗,A、B组注射等容量0.9%氯化钠注射液。D组在rt-PA注射后8、16、24h给予补阳还五汤29.69g/kg灌胃,A、B、C组给予等容量0.9%氯化钠注射液灌胃。灌胃治疗24h后,比较各组神经功能缺损严重程度、脑梗死体积、脑含水量及血清IL-6、TNF-α、MMP-9和MMP-2水平。结果 灌胃治疗24h后,C、D组大鼠神经功能缺损严重程度评分和脑梗死体积低于B组(P<0.05),D组亦低于C组(P<0.05)。与A组比较,B、C组脑含水量增加(P<0.05),D组与A组脑含水量比较无统计学差异(P>0.05)。C、D组脑含水量少于B组(P<0.05),D组亦少于C组(P<0.05)。B、C、D组血清IL-6、TNF-α、MMP-9和MMP-2水平均高于A组(P<0.05)。与B、C组比较,D组上述指标均降低(P<0.05)。结论 补阳还五汤对急性脑梗死大鼠有脑保护的作用,可改善神经功能缺损状态,减少脑梗死体积,减轻脑水肿,降低血清炎症因子水平。Objective To investigate the effects of Buyang Huanwu decoction on nerve function and serum inflammatory factors after thrombolysis with recombinant tissue plasminogen activator(rt-PA)in middle cerebral artery occlusion(MCAO)rats.Methods Twenty-four SPF grade male rats were randomly divided into four groups of sham operation group(group A),model control group(group B),rt-PA treatment group(group C)and Buyang Huanwu decoction+rt-PA treatment group(group D).After successful establishment of MCAO models in groups of B,C and D,the rats in groups of C and D were injected with rt-PA 10 mg/kg thrombolytic therapy via the tail vein.The rats in groups of A and B were injected with same volume of o.9%sodium chloride injection via tail vein.Twenty-four hours after gavage treatment,the severity of neurological impairment,cerebral infarction volume,cerebral water content and serum levels of IL-6,TNF-α,MMP-9 and MMP-2 were compared among all groups.Results After 24 hours of gavage treatment,the neurological impairment severity score and cerebral infarction volume in groups of C and D were lower than those in group B(P<0.05),and which in group D were also lower than those in group C(P<0.05).Compared with group A,the brain water content of groups of B and C was increased(P<o.05),and which was no statistical difference between groups of D and A(P>0.05).The brain water content of groups of C and D was less than that of group B(P<0.05),and which of group D was also less than that of group C(P<0.05).Serum levels of IL-6,TNF-α,MMP-9 and MMP-2 in groups of B,C and D were higher than those in group A(P<0.05).Compared with groups of B and C,the above indexes in group D were decreased(P<o.05).Conclusion Buyang Huanwu decoction has a brain protection in MCAO model rats,which may improve the status of neurological impairment,reduce the volume of cerebral infarction,alleviate cerebral edema,reduce serum levels of inflammatory factors.

关 键 词：补阳还五汤 急性脑梗死 溶栓治疗 

分 类 号：R277[医药卫生—中医学]