Vascular endothelial cellular mechanics under hyperglycemia and its role in tissue regeneration  

作　　者：Kui Wang Yongmei Ge Yongshuai Yang Zhenjian Li Jiayi Liu Yizebang Xue Yuanjun Zhang Xiangchao Pang A.H.W.Ngan Bin Tang 

机构地区：[1]Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen 518055,China [2]Department of Mechanical Engineering,University of Hong Kong,Hong Kong 999077,China [3]Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Shenzhen Key Laboratory of Cell Microenvironment,Shenzhen 518055,China [4]College of Materials Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China

出　　处：《Regenerative Biomaterials》2024年第5期49-58,共10页再生生物材料（英文版）

基　　金：supported by the National Key Research and Development Program of China grants(Project No.2019YFA0906004);the National Foundation of Science,Technology(Project No.11872200);Guangdong Provincial Key Laboratory of Advanced Biomaterials(Project No.2022B1212010003);the Shenzhen Science and Technology Innovation Committee(Project No.KCXFZ20211020174805009,JSGG20200225151916021);Presidential Foundation of Zhujiang Hospital(yzjj2022 ms08).

摘　　要：Diabetes is one of the most prevalent diseases worldwide.The tissue regeneration of diabetes patients is known to be rather tricky as the result of vascular dysfunction,and this leads to various clinical complications including diabetic foot ulcers.The vascular endothelial cells,which compactly line the inner surface of blood vessels,are responsible for the growth and maintenance of blood vessels and play an essential role in tissue regeneration.Although the mechanical properties of cells are generally known to be regulated by physiological/pathological conditions,few studies have been performed to investigate vascular endothelial cellular mechanics under hyperglycemia and the biological functions related to tissue regeneration.In this study,we conduct a systematic investigation of this issue.The results suggested that the stiffness of human umbilical vein endothelial cells(HUVECs)can be significantly regulated by the glucose concentration,subsequently,leading to significant alterations in cell migration and proliferation capabilities that are closely related to tissue regeneration.The rearrangement of the cytoskeleton induced by hyperglycemia through Cdc42 was found to be one of the pathways for the alteration of the cell stiffness and the subsequent cell dysfunctions.Therefore,we suggested that the inhibition of Cdc42 might be a promising strategy to facilitate various tissue regeneration for diabetes patients.

关 键 词：endothelial cell HYPERGLYCEMIA STIFFNESS MIGRATION CDC42 

分 类 号：R318[医药卫生—生物医学工程]