Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel  

作　　者：Delong Zhu Ying Hu Xiangkai Kong Yuansen Luo Yi Zhang Yu Wu Jiameng Tan Jianwei Chen Tao Xu Lei Zhu 

机构地区：[1]Department of Dermatology&Plastic Surgery,The Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China [2]Department of the Second Plastic Surgery,The First People’s Hospital of Foshan,Foshan 528000,China [3]Department of Research and Development,Huaqing Zhimei(Shenzhen)Biotechnology Co.,Ltd,Shenzhen 518107,People’s Republic of China [4]Center for Bio-Intelligent Manufacturing and Living Matter Bioprinting,Research Institute of Tsinghua University in Shenzhen,Tsinghua University,Shenzhen 518057,People’s Republic of China [5]Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,People’s Republic of China

出　　处：《Regenerative Biomaterials》2024年第5期106-120,共15页再生生物材料（英文版）

基　　金：supported by the Guangdong Basic and Applied Basic Research Foundation(2021A1515220120);the Natural Science Foundation of China(grant no.52075285).

摘　　要：Adipose mesenchymal stem cell(ADMSC)-derived exosomes(ADMSC-Exos)have shown great potential in regenerative medicine and been evidenced benefiting wound repair such as burns.However,the low yield,easy loss after direct coating,and no suitable loading system to improve their availability and efficacy hinder their clinical application for wound healing.And few studies focused on the comparison of biological functions between exosomes derived from different culture techniques,especially in exosome-releasing hydrogel system.Therefore,we designed a high-performance exosome controllable releasing hydrogel system for burn wound healing,namely loading 3D-printed microfiber culture-derived exosomes in a highly biocompatible hyaluronic acid(HA).In this project,we compared the biological functions in vitro and in a burn model among exosomes derived from the conventional two-dimensional(2D)plate culture(2D-Exos),microcarrier culture(2.5D-Exos),and 3D-printed microfiber culture(3D-Exos).Results showed that compared with 2D-Exos and 2.5D-Exos,3D-Exos promoted HACATs and HUVECs cell proliferation and migration more significantly.Additionally,3D-Exos had stronger angiogenesis-promoting effects in tube formation of(HUVECs)cells.Moreover,we found HA-loaded 3D-Exos showed better burn wound healing promotion compared to 2D-Exos and 2.5D-Exos,including accelerated burn wound healing rate and better collagen remodeling.The study findings reveal that the HA-loaded,controllable-release 3D-Exos repair system distinctly augments therapeutic efficacy in terms of wound healing,while concurrently introducing a facile application approach.This system markedly bolsters the exosomal loading efficiency,provides a robust protective milieu,and potentiates the inherent biological functionalities of the exosomes.Our findings provide a rationale for more efficient utilization of high-quality and high-yield 3D exosomes in the future,and a novel strategy for healing severe burns.

关 键 词：adipose-derived mesenchymal stem cells exosomes 3D microfiber culture hyaluronic acid hydrogel burn wound healing 

分 类 号：TQ427.26[化学工程]