Electrospun radially oriented berberine-PHBV nanofiber dressing patches for accelerating diabetic wound healing  

作　　者：Qiuyu Wang Sai Zhang Jiayi Jiang Shaojuan Chen Seeram Ramakrishna Wenwen Zhao Fan Yang Shaohua Wu 

机构地区：[1]College of Textiles&Clothing,Qingdao University,Qingdao 266071,China [2]College of Textile and Clothing,Dezhou University,Dezhou 253023,China [3]State Key Laboratory of Bio-Fibers and Eco-Textiles,Qingdao University,Qingdao 266071,China [4]Department of Mechanical Engineering,Center for Nanotechnology&Sustainability,College of Design and Engineering,National University of Singapore,Singapore 117576,Singapore [5]School of Basic Medicine,Qingdao University,Qingdao 266071,China

出　　处：《Regenerative Biomaterials》2024年第5期135-148,共14页再生生物材料（英文版）

基　　金：supported by State Key Laboratory of Bio-Fibers and Eco-Textiles of Qingdao University[TSKT202102];Startup Grant of Qingdao University;by the National Natural Science Foundation of China[22104010];Shandong Provincial Natural Science Foundation of China[ZR2021QB141];Qingchuang Science and Technology Plan of Shandong Province[2023KJ271].

摘　　要：A dressing patch made of radially oriented poly(3-hydroxybutyrateco-3-hydroxyvalerate)(PHBV)nanofibers was successfully manufactured with a modified electrospinning strategy.The aselectrospun PHBV radially oriented nanofiber dressing patch exhibited uniform and bead-free nanofibrous morphology and innovative radially oriented arrangement,which was demonstrated to possess obviously improved mechanical property,increased surface hydrophilicity and enhanced biological properties compared to the PHBV nanofiber dressing patch control with traditionally randomly oriented pattern.Interestingly,it was found that the radially oriented pattern could induce the cell migration from the periphery to the center along the radially oriented nanofibers in a rapid manner.To further improve the biofunction of PHBV radially oriented nanofiber dressing patch,berberine(Beri,an isoquinoline alkaloid)with two different concentrations were encapsulated into PHBV nanofibers during electrospinning,which were found to present a sustained drug release behavior for nearly one month.Importantly,the addition of Beri could impart the dressing patch with excellent anti-inflammatory property by significantly inhibiting the secretion of pro-inflammatory factors of M1 macrophages,and also showed an additive influence on promoting the proliferation of human dermal fibroblasts(HDFs),as well as inhibiting the growth of E.coli,S.aureus and C.albicans,compared with the Beri-free dressing patch.In the animal studies,the electrospun PHBV radially oriented nanofiber dressing patch loading with high Beri content was found to obviously accelerate the healing process of diabetic mouse full-thickness skin wound with shortened healing time(100%wound closure rate after 18 days’treatment)and improved healing quality(improved collagen deposition,enhanced re-epithelialization and neovascularization and increased hair follicles).In all,this study reported an innovative therapeutic strategy integrating the excellent physical cues of electrospun PHBV radially oriente

关 键 词：electrospinning radial alignment drug delivery wound dressing diabetic wound 

分 类 号：TB383[一般工业技术—材料科学与工程]