大肠杆菌通过诱导Akt磷酸化引起肌动蛋白微丝重新组装侵袭子宫内膜细胞  

E.coli invaded human endometrial cancer cells by cytoskeleton rearrangement through PI3K/AKT pathway

作　　者：程可 史俊秀 赵伟东[1] CHENG Ke;SHI Jun-xiu;ZHAO Wei-dong(Department of Development Cell Biology,College of Life Sciences,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学生命科学学院发育细胞生物学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2024年第5期518-522,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(31670845,31870832);沈阳市中青年科技创新人才支持计划(RC180089);辽宁省特聘教授;辽宁省教育厅青年科技人才“育苗”项目(QN2019028);中国医科大学师资博士后科研启动经费。

摘　　要：目的探究大肠杆菌感染时子宫内膜细胞细胞骨架的变化以及所涉及的信号通路。方法利用细菌侵袭实验分析大肠杆菌侵袭进入子宫内膜细胞的情况,以免疫荧光法观察大肠杆菌侵袭时子宫内膜细胞肌动蛋白微丝的变化,应用免疫印迹法以Akt磷酸化特异性抗体检测细胞内Akt的活化。结果大肠杆菌能够有效侵袭进入子宫内膜细胞;大肠杆菌的侵袭能够诱导子宫内膜细胞的肌动蛋白微丝发生重新组装、增强细胞内Akt磷酸化水平;Akt的抑制剂及其上游激酶PI3K的抑制剂均能维持肌动蛋白微丝的稳定并阻断大肠杆菌侵袭进入子宫内膜细胞。结论大肠杆菌通过诱导子宫内膜细胞中Akt分子发生磷酸化,引起肌动蛋白微丝发生重新组装,从而使细菌被内化至子宫内膜细胞内部。Objective To investigate the cytoskeleton changes of endometrial cells during E.coli infection and the involved signaling pathways.Methods Bacterial invasion assay was used to analyze the invasion of E.coli into endometrial cells.Immunofluorescence method was used to observe the changes of actin microfilaments in endometrial cells during the invasion of Escherichia coli,and Western blot method was used to detect the activation of Akt by Akt phosphorylation specific antibody.Results E.coli can effectively invade endometrial cells.The invasion of E.coli can induce the reassembly of actin microfilaments in endometrial cells and enhance the Akt phosphorylation level in the cells.Both Akt inhibitors and inhibitors of its upstream kinase PI3K maintain the stability of actin microfilaments and block the invasion of E.coli into endometrial cells.Conclusion E.coli induces phosphorylation of Akt molecule in endometrial cells,causing actin microfilaments to reassemble,thereby internalizing the bacteria into endometrial cells.

关 键 词：大肠杆菌 子宫内膜细胞 肌动蛋白 细菌侵袭 AKT磷酸化 

分 类 号：R711.32[医药卫生—妇产科学]