珊瑚状猴头菌多糖超声辅助提取工艺优化及抗结直肠癌活性研究  

Study on the Optimization of Ultrasonic-assisted Extraction Process of Hericium coralloides(Scop.)Pers.Polysaccharides and Its Anticolorectal Cancer Activity

作　　者：余金岂 邬柯奕 陈正睿 王婧涵 张鑫[2,3] 王春月 YU Jin-qi;WU Ke-yi;CHEN Zheng-rui;WANG Jing-han;ZHANG Xin;WANG Chun-yue(College of Plant Protection,Jilin Agricultural University,Changchun 130118;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118;College of Horticultural,Shenyang Agricultural University,Shenyang 110866)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]吉林农业大学食药用菌教育部工程研究中心,长春130118 [3]沈阳农业大学园艺学院,沈阳110866

出　　处：《生物技术通报》2024年第11期78-87,共10页Biotechnology Bulletin

基　　金：国家食用菌产业技术体系(CARS-20);国家级大学生科技创新训练计划(202310193047);天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006)。

摘　　要：【目的】珊瑚状猴头菌(Hericium coralloides(Scop.)Pers.)是一种食药用菌,重点优化其多糖(HCP)的超声提取方案,并对HCP的体外抗结直肠癌活性及机制进行探索。【方法】利用单因素实验结合响应面法(response surface methodology,RSM)对HCP超声提取条件进行优化。通过MTT实验与Western blot分析对HCP的抗结直肠癌活性与机制进行研究。【结果】珊瑚状猴头菌多糖超声提取的最优提取方案如下:提取功率417 W,液料比62 mL/g,提取时间52.5 min,多糖的提取率预测值为19.61%,实际提取率为(19.58±0.05)%。HCP通过降低SW480细胞中IKK、IκBα和NF-κB的磷酸化水平,减少了促炎因子IL-6的分泌,抑制SW480细胞生长,半数抑制浓度(half maximal inhibitory concentration,IC50)为1.246 mg/mL。【结论】珊瑚状猴头菌多糖的最佳超声辅助提取率为19.58%,HCP通过抑制NF-κB信号通路减少促炎细胞因子的分泌,进而抑制SW480细胞生长,对于结直肠癌具有潜在的治疗作用。【Objective】Hericium coralloides(Scop.)Pers.is a medicinal and edible fungus.This study focused on optimizing the ultrasonic extraction process of its polysaccharides(HCP)and exploring the in vitro anti-colorectal cancer activity and underlying mechanisms of HCP.【Method】Single-factor experiments combined with response surface methodology(RSM)S was used to optimize the ultrasonic extraction conditions of HCP.MTT assays and Western blot analyses were applied to investigate the anti-colorectal cancer activity and mechanisms of HCP.【Result】The optimal ultrasonic extraction conditions for H.coralloides polysaccharides were as follows:extraction power of 417 W,liquid-to-material ratio of 62 mL/g,and extraction time of 52.5 min.The predicted extraction yield of polysaccharides was 19.61%,with an actual yield of(19.58±0.05)%.HCP inhibited the growth of SW480 cells by reducing the phosphorylation levels of IKK,IκBα,and NF-κB,thereby decreasing the secretion of the pro-inflammatory factor IL-6.The half-maximal inhibitory concentration(IC50)was 1.246 mg/mL.【Conclusion】The optimal ultrasonic-assisted extraction rate of H.coralloides polysaccharides was 19.58%.HCP suppressed the secretion of pro-inflammatory cytokines by inhibiting the NF-κB signaling pathway,thus inhibiting the growth of SW480 cells.These findings suggest that HCP has potential therapeutic effects on colorectal cancer.

关 键 词：珊瑚状猴头菌 抗结直肠癌活性 超声提取 响应面法 多糖 NF-ΚB信号通路 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]