四尾栅藻提高大豆草甘膦抗性及促生效应分析  

Analysis of Increasing Glyphosate Resistance and Growth-promoting Effects in Soybean by Desmodesmus subspicatus

作　　者：李炯珊 杨泽 闫星 刘义珍 郭宇双 薛金爱[1,2] 孙希平 季春丽[1,2] 张春辉 李润植 LI Jiong-shan;YANG Ze;YAN Xing;LIU Yi-zhen;GUO Yu-shuang;XUE Jin-ai;SUN Xi-ping;JI Chun-li;ZHANG Chun-hui;LI Run-zhi(College of Agriculture,Shanxi Agricultural University,Taigu 030801;Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu 030801)

机构地区：[1]山西农业大学农学院,太谷030801 [2]山西省特用作物遗传与代谢工程研究中心,太谷030801

出　　处：《生物技术通报》2024年第11期236-247,共12页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31902344);国家重点研发计划部省联动项目(2021YFD1901100)。

摘　　要：【目的】表征四尾栅藻(Desmodesmus subspicatus)对草甘膦耐受性以及吸附或降解效应,评估施用四尾栅藻提高大豆(Glycine max)幼苗草甘膦抗性和促生作用以及清除根际草甘膦残留的效果,为解析微藻吸附或降解草甘膦等有机磷农药作用机制提供新思路。【方法】向四尾栅藻中施加不同剂量草甘膦,连续培养5 d,检测四尾栅藻生长,以及光合参数和培养液中草甘膦残留量。向大豆幼苗水培液中加入不同剂量草甘膦,连续培养7 d,检测大豆幼苗生长和光合参数。在草甘膦胁迫的大豆幼苗水培液中加入四尾栅藻藻液,连续培养14 d,检测大豆幼苗生理参数、草甘膦含量和水培液中草甘膦残留量。【结果】四尾栅藻对草甘膦有一定耐受性,在100 mg/L草甘膦胁迫下能维持有效生长。四尾栅藻连续培养5 d,培养液添加的草甘膦清除率达72.26%。草甘膦胁迫显著抑制大豆幼苗生长。在草甘膦胁迫下,施用四尾栅藻使大豆幼苗株高、根长、鲜重和干重分别比草甘膦胁迫的大豆幼苗最高提高15.98%、49.03%、49.20%和25.63%。相似地,大豆幼苗叶绿素荧光参数Y(II)、ETR和Fv/Fm值升高,以及NPQ值降低。与对照相比,施用四尾栅藻显著降低大豆幼苗和水培液中草甘膦残留26.3%和34.6%。【结论】四尾栅藻细胞可大量吸附或降解草甘膦。施用四尾栅藻能有效缓解草甘膦胁迫对大豆幼苗光合作用的损害,且能有效降低大豆幼苗对草甘膦的吸收和富集,进而促进大豆幼苗生长和阻滞草甘膦残留的迁移。【Objective】This study is to characterize the tolerance of Desmodesmus subspicatus,a microalga,to glyphosate as well as effects of adsorbing or degrading glyphosate.Also it is to evaluate the effects of D.subspicatus application on improving glyphosate resistance and promoting growth of soybean(Glycine max)seedlings,and removing glyphosate residues from rhizosphere.These findings may offer new insights into elucidation of the absorption or degradation mechanism of glyphosate and other organophosphorus pesticides by microalgae.【Method】Different dosages of glyphosate were added to the liquid medium,respectively,in which D.subspicatus was cultured for 5 d.Then microalgal growth and photosynthesis,together with glyphosate residue in the culture solution were examined.Glyphosate was added to the soybean seedling hydroponic solution for 7 d of cultivation,and then the growth and photosynthesis of soybean seedlings were measured.The algal cultures of D.subspicatus were added to the soybean seedling hydroponic solution for 14 d of cultivation under glyphosate stress.After that,physiological parameters were examined for the soybean seedlings,followed by analyzing levels of glyphosate residues in both the seedlings and hydroponic solution.【Result】The D.subspicatus had a certain degree of tolerance to glyphosate and maintained effective growth under 100 mg/L glyphosate stress.The removal rate of glyphosates added to the culture solution was high up to 72.26%after 5-day cultivation of the microalga.Glyphosate stress significantly inhibited soybean seedling growth.D.subspicatus application enhanced the plant height,root length,fresh weight and dry weight of soybean seedlings cultivated in the glyphosate-stressed solution by 15.98%,49.03%,49.20%and 25.63%,respectively,compared with the glyphosate-stressed controls.Similarly,values of chlorophyll fluorescence parameters Y(II),ETR and Fv/Fm increased while NPQ value decreased.Importantly,glyphosate residues in the soybean seedlings and the culture solution significant

关 键 词：四尾栅藻 大豆 草甘膦残留 抗逆性 促生效应 

分 类 号：S565.1[农业科学—作物学]