不同盐度培养下海洋真菌Aspergillus unguis DLEP2008001生物活性及次生代谢组变化  

Variation of Bioactivities and Secondary Metabolomics of Marine Fungus Aspergillus unguis DLEP2008001 Cultured under Different Salinities

作　　者：康晓博 张璟汐 卢甜甜 刘亚月 周龙建 张翼 KANG Xiao-bo;ZHANG Jing-xi;LU Tian-tian;LIU Ya-yue;ZHOU Long-jian;ZHANG Yi(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Marine Biomedicine Research&Development Center at Shenzhen Institute of Guangdong Ocean University,Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health,Zhanjiang 524088;Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang),Zhanjiang 524006;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034)

机构地区：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东省海洋生物制品工程实验室广东海洋大学深圳研究院海洋医药研发中心湛江市脑健康相关海洋药物与营养品重点实验室,湛江524088 [2]南方海洋科学与工程广东省实验室(湛江),湛江524006 [3]海洋食品精深加工关键技术省部共建协同创新中心大连工业大学,大连116034

出　　处：《生物技术通报》2024年第11期296-311,共16页Biotechnology Bulletin

基　　金：广东省基础与应用基础研究基金自然科学基金(面上项目)(2022A1515010783);广东省普通高校重点领域专项(生物医药与健康)(2021ZDZX2064);深圳市科创委基础研究面上项目(JCYJ20220530162014032);湛江市海洋青年人才创新项目(2022E05010);广东海洋大学大学生创新创业训练计划项目(S202110566014);广东海洋大学研究生教育创新计划项目(202232)。

摘　　要：【目的】以一株海洋真菌爪曲霉Aspergillus unguis DLEP2008001为对象,研究不同盐度对其生物活性和次生代谢产物的影响。【方法】对不同盐度培养下该菌株第14天和第28天的固体和液体培养基发酵产物提取物,分别采用薄层层析和抗氧化、乙酰胆碱酯酶抑制及抗菌生物活性自显影手段,分析其活性次生代谢产物差异,将样品进一步采用高效液相色谱以及基于液相色谱串联质谱的“多途径辅助的特征分子网络”代谢组学分析手段,系统地研究不同盐度培养下A.unguis DLEP2008001次生代谢产物的多样性变化。【结果】盐度可以调节抗氧化、抑制乙酰胆碱酯酶以及抗菌活性成分的产量与多样性,采用海水马铃薯蔗糖液体培养基28 d培养时在35 g/L盐度下出现最为丰富的特征代谢产物,而大米固体培养基在5 g/L盐度下特征代谢产物最为丰富、35 g/L盐度下也较丰富,固体发酵优势代谢产物的平均分子量随着盐度上升而总体呈现下降趋势,该菌株在高盐条件下的优势代谢产物多为含多羟基、多氨基的小分子化合物,推测可能参与渗透压调节。【结论】提取物盐度对该爪曲霉菌株的生物活性及次生代谢产物有显著的影响。【Objective】A marine fungus Aspergillus unguis DLEP2008001 was used for investigating the influence of salinity on the bioactivity and secondary metabolites.【Method】The crude extract samples of this strain cultured under different salinities in solid and liquid mediums for 14 and 28 days were analyzed by thin layer chromatography and antioxidative,acetylcholinesterase(AChE)inhibitory,and antimicrobial bioautographies to compare the bioactive secondary metabolites.The secondary metabolite diversity was further investigated by high performance liquid chromatography and“Multi-approach Assisted Feature Based Molecular Network(FBMN)”metabolomic analytical method based on liquid chromatography-tandem mass spectrometry(LC-MS/MS).【Result】The salinity regulated the yield and diversity of antioxidant,AChE inhibitory ingredients and anti-microbial.In seawater-potato-sucrose liquid medium,the strain presented the most fertile feature metabolites when cultured for 28 d under salinity of 35 g/L,while in rice solid medium,it produced the most abundant predominant compounds under salinity of 5 g/L and 35 g/L as the second best condition.The average molecular weights of the predominant metabolites from solid culture generally deceased with the ascending of the salinity and they were mostly small molecules with multiple hydroxyl and amino groups,which are speculated to involve the adjustment of osmotic pressure.【Conclusion】Salinity exerts a significant influence on the bioactivity and secondary metabolites of this strain.

关 键 词：不同盐度 海洋真菌 分子网络 次生代谢产物 生物活性 

分 类 号：Q936[生物学—微生物学]