辅助生殖患者卵泡液中SCF、SDF-1及脂溶性维生素水平与优质胚胎数及妊娠结局的关系  

Correlation of Follicular SCF,SDF-1 and Fat-soluble Vitamins with the Number of High-Quality Embryos and Outcome of in Vitro Fertilization-Embryo Transfer

作　　者：刘芳 唐大伟 白刚 马文叶 王国平 LIU Fang;TANG Dawei;BAI Gang;MA Wenye;WANG Guoping(Reproductive Center of Yinchuan Maternal and Child Health Hospital,Yinchuan 750001,China)

机构地区：[1]银川市妇幼保健院生殖中心,银川750001

出　　处：《宁夏医科大学学报》2024年第11期1132-1136,共5页Journal of Ningxia Medical University

基　　金：银川市科技局科技创新重大重点专项(2021-SF-008);宁夏回族自治区重点研发计划项目(2021BEG03041)。

摘　　要：目的研究辅助生殖患者卵泡液中干细胞因子(SCF)、基质细胞衍生因子-1(SDF-1)及脂溶性维生素水平与优质胚胎数及结局的关系。方法选取105例行体外受精-胚胎移植(IVF-ET)辅助生殖患者,根据有无妊娠分为妊娠组与未妊娠组。收集患者临床资料,检测取卵日的基础性激素水平,采用酶联免疫吸附法(ELISA)检测卵泡液中SCF、SDF-1及维生素D水平,高效液相色谱法(HPLC)检测卵泡液中维生素A、E水平。采用Pearson分析卵泡液SCF、SDF-1及脂溶性维生素水平与优质胚胎数相关性,Logistic回归方程分析临床妊娠的影响因素。结果妊娠组优质胚胎数、卵泡液中SCF、SDF-1、维生素A、D、E水平均高于未妊娠组(P均<0.05)。Pearson分析显示,卵泡液中SCF、SDF-1、维生素A、D、E水平与优质胚胎数呈正相关(P均<0.05)。多元线性回归显示,排除年龄、BMI、不孕时间、Gn天数与总量、获卵数、胚胎数因素后,卵泡液中SCF、SDF-1、维生素A、D、E水平仍与优质胚胎数有关(P均<0.05)。Logistic回归分析显示,优质胚胎数、SCF、SDF-1、维生素A、D、E水平是临床妊娠的影响因素(P均<0.05)。结论卵泡液中SCF、SDF-1及脂溶性维生素水平与胚胎发育密切相关,且其水平升高有助于促进临床妊娠,可为评估辅助生殖者早期IVF-ET结局提供参考。Objective To study the correlation of follicular stem cell factor(SCF),stromal cell derived factor-1(SDF-1)and fat-soluble vitamins with the number of high-quality embryos and outcome of in vitro fertilization-embryo transfer(IVF-ET).Methods A total of 105 patients underwent IVF-ET were enrolled,and classified into two groups according to their pregnancy outcomes,pregnant group and non-pregnant group.The clinical data of patients were collected.The sex hormone levels were detected on the day of retrieving ova.Levels of SCF,SDF-1 and vitamin D in follicular fluid were detected by enzyme-linked immunosorbent assay.High performance liquid chromatography was used to detect the levels of vitamin A and E in follicular fluid.Pearson analysis was used to discuss the correlation between the levels of SCF,SDF-1,and fat-soluble vitamins in follicular fluid and the number of high-quality embryos.Logistic regression equation was used to screen the influencing factors of clinical pregnancy.Results The number of high-quality embryos and the levels of SCF,SDF-1 and vitamin A,D and E in follicular fluid were higher in pregnant group than in non-pregnant group(P all<0.05).Pearson analysis showed that the levels of SCF,SDF-1,vitamin A,D and E in follicular fluid were positively correlated with the number of quality embryos(P<0.05).Multiple linear regression showed that the levels of SCF,SDF-1,vitamins A,D and E in follicular fluid were still associated with the number of quality embryos after excluding the factors of age,body mass index(BMI),duration of infertility,days and total number of gonadotropin(Gn),number of eggs obtained,and number of embryos(P all<0.05).Logistic regression analysis denoted that the number of high-quality embryos,SCF,SDF-1,vitamin A,D,and E levels were all influential factors for clinical pregnancy(P all<0.05).Conclusion The levels of SCF,SDF-1 and fat-soluble vitamins in follicular fluid are closely related to embryonic development,and their elevated levels are beneficial to clinical pregnancy,providing a

关 键 词：辅助生殖 干细胞因子 基质细胞衍生因子-1 脂溶性维生素 优质胚胎 妊娠结局 

分 类 号：R714[医药卫生—妇产科学]