Structural characterization and mechanisms of macrophage immunomodulatory activity of a novel polysaccharide with a galactose backbone from the processed Polygonati Rhizoma  

作　　者：Hongna Su Lili He Xina Yu Yue Wang Li Yang Xiaorui Wang Xiaojun Yao Pei Luo Zhifeng Zhang 

机构地区：[1]Faculty of Chinese Medicine,State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Macao,999078,China [2]Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China,Southwest Minzu University,Chengdu,610041,China [3]Centre for Artificial Intelligence Driven Drug Discovery,Faculty of Applied Sciences,Macao Polytechnic University,Macao,999078,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第7期1076-1090,共15页药物分析学报（英文版）

基　　金：supported by Sichuan Provincial Regional Innovation Cooperation Project,China(Grant No.:2023YFQ0084);the Macao Science and Technology Development Fund(FDCT),China(Grant No.:0043/2021/AGJ).

摘　　要：A purified polysaccharide with a galactose backbone(SPR-1,Mw 3,622 Da)was isolated from processed Polygonati Rhizoma with black beans(PRWB)and characterized its chemical properties.The backbone of SPR-1 consisted of[(4)-b-D-Galp-(1]9/4,6)-b-D-Galp-(1/4)-a-D-GalpA-(1/4)-a-D-GalpA-(1/4)-aD-Glcp-(1/4,6)-a-D-Glcp-(1/4)-a/b-D-Glcp,with a branch chain of R1:b-D-Galp-(1/3)-b-D-Galp-(1/connected to the/4,6)-b-D-Galp-(1/via O-6,and a branch chain of R2:a-D-Glcp-(1/6)-a-D-Glcp-(1/connected to the/4,6)-a-D-Glcp-(1/via O-6.Immunomodulatory assays showed that the SPR-1 significantly activated macrophages,and increased secretion of NO and cytokines(i.e.,IL-1b and TNF-a),as well as promoted the phagocytic activities of cells.Furthermore,isothermal titration calorimetry(ITC)analysis and molecular docking results indicated high-affinity binding between SPR-1 and MD2 with the equilibrium dissociation constant(KD)of 18.8 mM.It was suggested that SPR-1 activated the immune response through Toll-like receptor 4(TLR4)signaling and downstream responses.Our research demonstrated that the SPR-1 has a promising candidate from PRWB for the TLR4 agonist to induce immune response,and also provided an easily accessible way that can be used for PR deep processing。

关 键 词：Polygonati Rhizoma POLYSACCHARIDE Structural properties Immunoregulatory 

分 类 号：R96[医药卫生—药理学]