呼吸道合胞病毒B亚型感染特性研究  

Infectiousness of isolates of respiratory syncytial virus subtype B strains

作　　者：唐维 张蓉蓉 陈奇[2] 叶青 黄升海[1] TANG Wei;ZHANG Rongrong;CHEN Qi;YE Qing;HUANG Shenghai(School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,Anhui,China;State Key Laboratory of Pathogen and Biosecurity,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100071,China)

机构地区：[1]安徽医科大学基础医学院,合肥230032 [2]军事科学院军事医学研究院病原微生物生物安全全国重点实验室,北京100071

出　　处：《军事医学》2024年第10期730-736,共7页Military Medical Sciences

基　　金：北京市自然科学基金-海淀原始创新联合基金(L222056)。

摘　　要：目的明确呼吸道合胞病毒(RSV)B亚型18537株在不同宿主细胞系上的感染特征,并评价其在不同动物模型中的致病性及病理损伤特点,为RSV感染及致病机制研究提供实验与技术基础。方法通过评价感染细胞病变特征、病毒蚀斑形态、病毒蛋白表达以及体外增殖效率等,明确其基本生物学特征;通过滴鼻途径感染10月龄BALB/c小鼠和6周龄棉鼠,检测感染后肺组织中的病毒载量,并分析病理损伤特征,完成其致病性评价。结果RSV B亚型18537株导致Hep-2细胞出现多细胞核融合性病变,在BHK-21细胞中可以形成典型的病毒蚀斑;同时,在Hep-2和A549细胞上可检测到病毒蛋白的表达。滴鼻途径感染BALB/c小鼠和棉鼠,感染后第5天可在肺组织中检测到病毒核酸,同时该剂量可造成肺组织肺泡壁轻度增厚,伴有散在的淋巴细胞与中性粒细胞浸润。结论RSV B亚型18537株可在Hep-2和A549细胞上有效增殖,并能感染BALB/c小鼠和棉鼠,导致肺组织出现一定程度的病理损伤。通过比较发现,相比A亚型A2株,RSV B亚型18537株在细胞和动物中的感染能力较弱。Objective To determine the infectiousness of the respiratory syncytial virus(RSV)18537 strain of subtype B in different host cell lines and evaluate its pathogenicity and pathological damage in various animal models.Methods The cytopathic features,viral plaque morphology,viral protein expression,and in vitro proliferation efficiency were assessed to determine the basic biological characteristics of such infections.Nasal drops were used to infect 10-month-old BALB/c mice and 6-week-old cotton mice.The viral load in lung tissue after infection was detected,and the pathological injury was analyzed to assess the pathogenicity.Results The RSV 18537 strain of subtype B strain induced polynuclear fusion in Hep-2 cells,and typical viral plaques were formed in BHK-21 cells.In addition,viral proteins could be detected in Hep-2 and A549 cells.In BALB/c and cotton mice infected with nasal drops,viral nucleic acids were detectable in lung tissue on day 5 post-infection.This dose caused mild thickening of alveolar walls with scattered lymphocytes and neutrophil infiltration.Conclusion The RSV 18537 strain of subtype B can be effectively proliferated in Hep-2 and A549 cells while infecting BALB/c mice and cotton mice,resulting in pathological injury to lung tissue.The 18537 strain of RSV subtype B is less contagious than the A2 strain of subtype A both in cells and animals.

关 键 词：呼吸道合胞病毒 B亚型 HEP-2细胞 病毒感染特征 病毒致病性 

分 类 号：R373.1[医药卫生—病原生物学]