人感染札如病毒的遗传多样性与重组事件分析  

Genetic diversity and recombination events of human infections with Sapovirus

作　　者：刘婉秋 胡明达 胡晓丰 任洪广 王辛 何雅青 LIU Wanqiu;HU Mingda;HU Xiaofeng;REN Hongguang;WANG Xin;HE Yaqing(School of Public Health,University of South China,Hengyang,Hunan 421001,China;Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100071,China;Chinese PLA Center for Disease Control and Prevention,Beijing 100071,China;Institute of Pathogen Microbiology,Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong 518055,China)

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001 [2]军事科学院军事医学研究院,北京100071 [3]中国人民解放军疾病预防控制中心,北京100071 [4]深圳市疾病预防控制中心,病原微生物研究所,广东深圳518055

出　　处：《军事医学》2024年第10期737-743,共7页Military Medical Sciences

基　　金：国家自然科学基金项目(32070025,62206309);病原微生物生物安全国家重点实验室研究项目(SKLPBS2214,SKLPBS2234)。

摘　　要：目的利用生物信息学技术,系统研究全球范围内人感染札如病毒(SaV)的遗传特征和重组现象。方法从美国国家生物技术信息中心(NCBI)下载全球SaV的全基因组数据,并筛选出序列质量较高的毒株作为实验样本。构建SaV的分子进化树进行遗传特征分析,进一步使用重组分析软件对人感染的4种SaV基因型(GⅠ、GⅡ、GⅣ和GⅤ)进行重组事件分析。结果SaV在全球范围内存在较大的遗传多样性,感染宿主谱广泛。人类感染的SaV主要是GⅠ、GⅡ、GⅣ和GⅤ型,其中GⅤ型为人猪共有。SaV的重组分析显示,GⅡ型重组频次较高,GⅤ型在不同宿主间发生重组。病毒的重组集中发生在主要病毒蛋白(VP1)和次要病毒蛋白(VP2)区。结论通过对人感染SaV的遗传特征进行系统分析,确定了基因型的分布和流行情况,揭示SaV重组规律,突显了SaV的遗传动态,为了解病毒流行趋势和制定防控策略提供重要参考。Objective To investigate the genetic characteristics and recombination of human-infected sapoviruses(SaVs)worldwide using bioinformatics.Methods The complete genome sequences of SaVs were downloaded from the National Center for Biotechnology Information(NCBI)while high-quality complete genomes were retained for analysis.Molecular phylogenetic trees of SaVs were constructed to analyze their genetic characteristics,followed by recombination analysis of human-infected SaV strains genetype Ⅰ,Ⅱ,Ⅳ,and Ⅴ(GⅠ,GⅡ,GⅣ,and GⅤ)with recombination analysis software.Results SaVs exhibited substantial genetic diversity worldwide and infected a wide range of hosts.Humanassociated SaVs included GⅠ,GⅡ,GⅣ,and GⅤ,with GⅤ shared between human and swine hosts.Genetype recombination analysis of SaVs revealed a high frequency of recombination in SaV GⅡ strains that involved diverse hosts in the field of SaV GⅤ strains.Recombination breakpoints of the virus were concentrated in the major viral proteins 1(VP1)and minor viral proteins 2(VP2).Conclusion Based on systematic analysis of the genetic characteristics of human-infected SaVs,the genotype distribution and prevalence of SaVs are investigated,the recombination patterns of SaV revealed,and its genetic dynamics highlighted.These findings can offer insights into epidemiological trends of viruses and help devise effective prevention and control strategies.

关 键 词：札如病毒 系统发育树 遗传重组 遗传多样性 分子进化 

分 类 号：R373[医药卫生—病原生物学]