Discovery of potent anti-MRSA components from Dalbergia odorifera through UPLC-Q-TOF-MS and targeting PBP2a protein through in-depth transcriptomic,in vitro,and in-silico studies  

作　　者：Jiajia Wu Syed Shams ul Hassan Xue Zhang Tao Li Abdur Rehman Shikai Yan Huizi Jin 

机构地区：[1]Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs,School of Pharmacy,Shanghai Jiao Tong University,Shanghai,200240,China [2]Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai,200241,China [3]College of Life Sciences,Northwest A&F University,Yangling,Shaanxi,712100,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第8期1251-1254,共4页药物分析学报（英文版）

基　　金：the National Natural Science Foundation of China(Grant No.:81973191);Shanghai Agriculture Applied Technology Development Program,China(Program No.:T20200104);the National Key Research and Development Program of China(Program No.:2018YFE0192600)for providing financial support for the complete undertaking.

摘　　要：Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agents.Approximately 119 compounds from 90 plants were recognized as potent anti-bacterials[2].Dalbergia odorifera,a traditional Chinese plant,has demonstrated anti-tumor,anti-microbial,anti-inflammatory,and cardiovascular protective effects[3].Limited studies have explored D.odorifera flavonoids'inhibitory activity against MRSA.Transcriptomics,a high-throughput method,aided in comprehending plant antibacterial therapy by generating data for gene expression,target identification,and pathway analysis[4].Consequently,our study aimed to assess D.odorifera's anti-MRSA effects and reveal its material foundation and antibacterial mechanism by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS),and transcriptomic analysis,in vitro,and in-silico studies.

关 键 词：protective MRSA anti 

分 类 号：R285.5[医药卫生—中药学]