亚致死浓度虫螨腈对番茄潜叶蛾生长发育及酶活的影响  

Effects of sublethal concentrations chlorfenapyr on growth,development and enzyme activity of Tuta absoluta

作　　者：李冬桂 彭晨 马睿馨 陈亚平 桂富荣[1] 孙仲享 LI Dong-Gui;PENG Chen;MA Rui-Xin;CHEN Ya-Ping;GUI Fu-Rong;SUN Zhong-Xiang(State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan,Plant Protection College of Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院,云南生物资源保护与利用国家重点实验室,昆明650201

出　　处：《环境昆虫学报》2024年第6期1349-1357,共9页Journal of Environmental Entomology

基　　金：国家重点研发计划(2021YFD1400200);云南省教育厅资助项目(2023Y1050)。

摘　　要：为明确虫螨腈对番茄潜叶蛾Tuta absoluta亲代(F_(0))及子一代(F_(1))的亚致死效应,以及探究番茄潜叶蛾F_(0)和F_(1)中解毒酶和保护酶系对亚致死浓度虫螨腈的响应情况,本研究采用浸叶法测定虫螨腈对番茄潜叶蛾的毒力,通过生物测定分析了亚致死浓度虫螨腈LC_(25)对番茄潜叶蛾F_(0)和F_(1)生长发育的影响,并测定了虫螨腈LC_(25)浓度处理后番茄潜叶蛾体内的解毒酶系和保护酶系的活力变化。研究发现,虫螨腈对番茄潜叶蛾2龄幼虫的生物活性较高,LC_(50)为1.33 mg/L。亚致死浓度虫螨腈LC_(25)处理后番茄潜叶蛾F_(0)产卵量显著下降,F_(1)的蛹重显著降低,蛹期和成虫前期显著延长。在解毒酶方面,亚致死浓度的虫螨腈对番茄潜叶蛾F_(0)的谷胱甘肽-S-转移酶(GST)和多功能氧化酶(MFO)表现为抑制作用,羧酸酯酶(CarE)没有明显变化;虫螨腈LC_(25)浓度对F_(1)的GST和MFO有诱导作用,其中GST诱导作用最强,而CarE作用不明显。在保护酶方面,亚致死浓度的虫螨腈仅对F_(0)番茄潜叶蛾过氧化氢酶(CAT)有诱导作用,而超氧化物歧化酶(SOD)和过氧化物酶(POD)无变化;虫螨腈LC_(25)浓度对F_(1)的CAT、POD和SOD作用不明显。虫螨腈LC_(25)浓度显著影响番茄潜叶蛾的生长发育和繁殖,且能显著影响其体内部分解毒酶和保护酶的活性。因此,本研究为促进合理使用虫螨腈防治番茄潜叶蛾提供了有价值的参考。In order to evaluate the sublethal effects of chlorfenapyr on F_(0)generation and progeny(F_(1)generation)of Tuta absoluta and to explore the response of detoxification enzymes and protective enzymes in F_(0)and F_(1)of T.absoluta to chlorfenapyr.This study used the leaf soaking method to determine the toxicity of chlorfenapyr to T.absoluta,and the sublethal effects of LC_(25)exposure to chlorfenapyr on growth and development of F_(0)and F_(1)generations of T.absoluta were analyzed by bioassay and the changes on F_(0)generation and progeny(F_(1)generation)of T.absoluta of detoxification enzymes and protective enzymes in the body after LC_(25)treatment were determined.The results showed that the activity of chlorfenapyr to the 2nd instar larvae of T.absoluta was high with the LC_(50)value of 1.33 mg/L.The number of eggs laid in the sublethal pesticide concentrations LC_(25)chlorfenapyr significantly reduced of F_(0)generation.Exposure to sublethal pesticide concentrations reduced the weight of F_(1)pupae,significantly prolonged the pupal period and total pre-oviposition period.In terms of detoxification enzymes,there was no significant change in carboxylesterase(CarE)activity,but glutathione S-transferase activity(GSTs)and Mixed-function oxidase(MFO)were distinctly inhibited by sublethal concentrations of chlorfenapyr on F_(0)generation.There was no significant change in CarE activity compared with the control,but GSTs and MFO were distinctly increased by sublethal concentrations of chlorfenapyr on F_(1).In terms of protective enzymes,the sublethal concentration of chlorfenapyr had no significant affect on the activities of POD and SOD on F_(0)generation in T.absoluta,while there was inducedeffect on the activity of CAT and the three protective enzymes CAT,POD and SOD on F_(0)generation.In summary,chlorfenapyr at the sublethal concentrations could significantly affect the growth and development of T.absoluta and the activity of some detoxification enzymes and protective enzymes in vivo.Therefore,this study provide

关 键 词：番茄潜叶蛾 虫螨腈 亚致死效应 解毒酶 保护酶 

分 类 号：Q965.9[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]