稀土氧化钐尘肺病工人血清蛋白质组学特征研究  

Study on serum proteomic characteristics of workers with rare earth samarium oxide pneumoconiosis

作　　者：王晓晖 丁锐黠 马腾 毕艳楠 阴海静[1] Wang Xiaohui;Ding Ruixia;Ma Teng;Bi Yannan;Yin Haijing(School of Public Health,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China;School of Basic Medicine and Forensic Medicine,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China)

机构地区：[1]内蒙古科技大学包头医学院公共卫生学院,包头014040 [2]内蒙古科技大学包头医学院基础医学与法医学院,包头014040

出　　处：《中华劳动卫生职业病杂志》2024年第11期807-814,共8页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：内蒙古自治区自然科学基金项目(2020BS08004,2024MS08087);包头医学院青苗计划(BYJJ-ZRQM202316);包头医学院博士科研启动基金项目(BSJJ201812);2022年包头市青年创新人才项目。

摘　　要：目的筛选稀土氧化钐尘肺病工人血清中的差异蛋白质,为寻找其早期诊断生物标志物提供新思路。方法于2019年4月,选择内蒙古自治区包头市某稀土厂确诊氧化钐尘肺病的3名男性工人作为观察组,未接触粉尘的3名男性工人作为对照组。通过Label-free蛋白质组学方法对其血清进行测序,筛选出差异蛋白质,并进行蛋白质相邻类聚簇(cluster of orthologous groups of proteins,COG)注释、基因本体(gene ontology,GO)富集分析,以及京都基因和基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路分析。运用相互作用基因库检索工具数据库和Cytoscape 3.9.1软件绘制蛋白质-蛋白质相互作用网络,CytoHubba插件筛选出评分较高的差异蛋白质,采用实时荧光定量聚合酶链式反应(real-time fluorescence quantitative polymerase chain reaction,RT/q-PCR)对蛋白质组测序结果进行验证。结果稀土氧化钐尘肺病工人血清中共筛选出45个上调差异蛋白质和5个下调差异蛋白质,COG功能分类中数量较多的是翻译后修饰、蛋白质周转、伴侣;GO富集到补体激活(经典途径)等生物过程条目25条,细胞外重组素等细胞组分条目15条,蛋白质结合等分子功能条目10条;KEGG富集分析到的通路主要为传染性疾病、免疫系统、信号转导和免疫性疾病。得分前10的蛋白质为结合珠蛋白、补体子组分C1R、补体子组分C1S、载脂蛋白C-Ⅲ、载脂蛋白A-Ⅱ、凝血酶原、α清蛋白、补体成分C8γ链、补体成分C6、补体成分C7。RT/q-PCR验证结果显示,观察组结合珠蛋白、凝血酶原和补体子组分C1S基因表达水平均明显高于对照组,差异均有统计学意义(P<0.05)。结论筛选出稀土氧化钐尘肺病工人血清中10个差异表达的蛋白质,为氧化钐尘肺病早期诊断的生物标志物筛选提供新思路。ObjectiveTo screen differential proteins in the serum of workers with rare earth samarium oxide pneumoconiosis,in order to provide new ideas for finding its early diagnostic biomarkers.MethodsIn April 2019,three male workers diagnosed with samarium oxide pneumoconiosis at a rare earth factory in Baotou City,Inner Mongolia Autonomous Region were selected as the observation group,and three male workers who were not exposed to dust were selected as the control group.The serum was sequenced using the Label-free proteomic method to screen for differentially expressed proteins,followed by cluster of orthologous groups of proteins(COG)annotation,gene ontology(GO)enrichment analysis,and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis.The interaction gene library retrieval tool database and Cytoscape 3.9.1 software were used to draw protein-protein interaction networks.CytoHubba plugin was used to screen for differentially expressed proteins with high scores,and real-time fluorescence quantitative polymerase chain reaction(RT/q-PCR)was used to validate the proteomic sequencing results.ResultsA total of 45 up-regulated differentially expressed proteins and 5 down-regulated differentially expressed proteins were screened out in the serum of workers with rare earth samarium oxide pneumoconiosis.In the COG functional classification,post-translational modifications,protein turnover,and chaperones were the most numerous.GO enrichment included 25 entries for biological processes such as complement activation(classical pathways),15 entries for cellular components such as extracellular recombinants,and 10 entries for molecular functions such as protein binding.The pathways identified by KEGG enrichment analysis mainly included infectious diseases,immune system,signal transduction,and immune related diseases.The top 10 scoring proteins were haptoglobin,complement C1r subcomponent,complement C1s subcomponent,apolipoprotein C-Ⅲ,apolipoprotein A-Ⅱ,prothrombin,afamin,complement component C8 gamma chain,complement comp

关 键 词：尘肺 氧化钐 蛋白质组学 稀土 

分 类 号：R135.2[医药卫生—劳动卫生]