分级醇沉败酱草多糖的理化特征及抑制糖消化酶活性研究  

Physicochemical characteristics of graded alcohol precipitation Herba Patriniapolisa Calid and inhibition of sugar-digesting enzyme activity

作　　者：魏赛雪 惠和平 郭栋费 张心驰 郭敏 李晓东[1,3,4] Wei Saixue;Hui Heping;Guo Dongfei;Zhang Xinchi;Guo Min;Li Xiaodong(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China;Institute of Chinese Materia Medica,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Professional Technical Center for Secondary Exploitation and Clinical Evaluation of Longyao Large Varieties,Gansu Academy of Traditional Chinese Medicine,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]商洛学院生物医药与食品工程学院,陕西商洛726000 [3]甘肃省中医院中药研究所,甘肃兰州730050 [4]甘肃省中医药研究院陇药大品种二次开发及临床疗效评价行业技术中心,甘肃兰州730050

出　　处：《兰州大学学报（医学版）》2024年第11期8-16,共9页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省高等学校创新基金资助项目(2021A-260);国家自然科学基金资助项目(82160818);甘肃省中医药管理局科研资助项目(GZKP-2023-3)。

摘　　要：目的探究不同分级败酱草多糖的理化特征、糖消化酶抑制活性以及两者的相关性。方法采用热水提取、不同浓度乙醇(50%、60%、70%、80%)醇沉得到4种败酱草多糖HP-50、HP-60、HP-70和HP-80,使用苯酚-硫酸法、气相色谱、高效凝胶渗透色谱和紫外分光度法分别测定多糖的含量、单糖组成、分子量等理化特征以及对α-淀粉酶和α-葡萄糖苷酶抑制活性,运用双变量相关性分析比较4种多糖抑制糖消化酶的活性与其理化特征的相关性。结果所有败酱草多糖均表现出浓度依赖性的糖消化酶抑制活性,其中HP-80的糖消化酶抑制活性最好;与其他醇沉浓度相比,HP-80葡萄糖摩尔分数较高,鼠李糖和阿拉伯糖摩尔分数较低,相对分子量较小。结论败酱草多糖相对分子量为50~100 kDa、鼠李糖摩尔分数在9.83%以下、阿拉伯糖与葡萄糖物质的量比小于1更有利于败酱草多糖抑制α-淀粉酶和α-葡萄糖苷酶的活性。Objective To investigate the physicochemical characteristics and glycodigestive enzyme inhibitory activity of different fractions of Herba patriniae polysaccharides,as well as the correlation between them.Methods Four kinds of HPs(HP-50,HP-60,HP-70 and HP-80)were obtained by hot water extraction and alcohol precipitation at different concentrations of ethanol(50%,60%,70%,80%),and the content and monosaccharide composition of polysaccharides determined by phenol-sulfuric acid method,gas chromatography(GC),high performance liquid chromatography(GPC)and ultraviolet spectrophotometry(UV).The activities of four polysaccharides in inhibiting sugar-digesting enzymes were compared via a bivariate correlation analysis regarding their physical and chemical characteristics such as molecular weight and inhibitory activity againstα-amylase andα-glucosidase.Results All HPs exhibited concentration-dependent inhibition of sugardigesting enzymes,and HP-80 had the best inhibitory activity of sugar-digesting enzymes.Compared with other alcohol precipitation concentrations,HP-80 had a higher glucose content,rhamnose and arabinose content was lower,and the molecular weight smaller.Conclusion It is speculated that the relative molecular weight of Herba patriniae polysaccharides is 50~100 kDa,the molar fraction of rhamnose less than 9.83%and the amount ratio of arabinose to glucose substance less than 1,which could more conducive to the inhibition ofα-amylase andα-glucoside activities of HPs.

关 键 词：分级醇沉 败酱草多糖 α-淀粉酶抑制活性 α-葡萄糖苷酶抑制活性 相关性分析 

分 类 号：R285.5[医药卫生—中药学]