短柄乌头中二萜生物碱类成分及心脏毒性研究  

Study on Diterpenoid Alkaloids from Aconitum Brachypodum and Their Cardiotoxicity

作　　者：陶培 刘丛改 红花 拉巴次仁 陈佳佳 郑清洋 泽翁拥忠[1] 王毓杰[1] TAO Pei;LIU Conggai;HONG Hua;Labaciren;CHEN Jiajia;ZHENG Qingyang;Zewengyongzhong;WANG Yujie(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Yongchuan District Hospital of Traditional Chinese Medicine,Chongqing 402160,China;Nyingchi Hospital of Tibetan Medicine,Nyingchi 860000,China;Nyingchi Gongbo'gyamda County Jiangda Township Health Center,Nyingchi 860000,China)

机构地区：[1]成都中医药大学,成都611137 [2]重庆市永川区中医院,重庆402160 [3]林芝市藏医院,西藏林芝860000 [4]林芝市工布江达县江达乡卫生院,西藏林芝860000

出　　处：《中国现代应用药学》2024年第18期2572-2579,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：四川省自然科学基金项目(2024NSFSC0702);四川省中医药管理局科学技术研究专项课题(2024MS178)。

摘　　要：目的 研究短柄乌头块根中二萜生物碱的结构及心脏毒性。方法 采用硅胶柱色谱及波谱法分离鉴定化合物的结构;采用大鼠模型比较不同化合物的心脏毒性。结果 从短柄乌头中分离得到12个二萜生物碱,分别鉴定为secokaraconitine(1)、oxonitine(2)、查斯曼宁(3)、草乌甲素(4)、尼奥林(5)、去氧乌头碱(6)、乌头碱(7)、印乌碱(8)、滇乌碱(9)、塔拉萨敏(10)、苯甲酰乌头原碱(11)、乌头原碱(12)。通过整体动物心脏毒性试验证实:1-OH、3-OH、8-COCH_(3)、14-苯甲酰基能够增强乌头烷类化合物的心脏毒性,6-OCH_(3)、N-CHO、N=C能够降低该类化合物的心脏毒性。结论通过整体动物试验明确了不同取代基对乌头烷类化合物心脏毒性的影响。OBJECTIVE To study the sturcture and cardiotoxicity of diterpenoid alkaloids from the roots of Aconitum brachypodum.METHODS Silica gel column chromatography and spectroscopy was used to separate and identify the structures of compounds.The cardiotoxicity of different compounds were compared using a rat model.RESULTS Twelve diterpenoid alkaloids were isolated and identified as secokaraconitine(1),oxonitine(2),chasmanine(3),crassicauline A(4),neoline(5),deoxyaconitine(6),aconitine(7),indaconitine(8),yunaconitine(9),talatisamine(10),benzoylaconine(11),and aconine(12).Through cardiotoxicity assay,it had been confirmed that 1-OH,3-OH,8-COCH_(3),and 14-benzoyl group could increase cardiotoxicity of aconitane compounds,while 6-OCH_(3),N-CHO and N=C could reduce it.CONCLUSION The effects of different substituents on the cardiotoxicity of aconitane compounds are clarified through overall animal experiments.

关 键 词：乌头属 短柄乌头 二萜生物碱 心脏毒性 secokaraconitine oxonitine 

分 类 号：R285.5[医药卫生—中药学]