玉屏风散改善肺巨噬细胞焦亡的研究  

Research of Yupingfeng powder regulating to improve pulmonary macrophage cells pyroptosis

作　　者：何莹莹 王丽娜[3] 孙德兴 贾志财 李倩 HE Ying-ying;WANG Li-na;SUN De-xing;JIA Zhi-cai;LI Qian(School of Public Health,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Clinical College of Traditional Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Department of Occupational Disease,The Third Affiliated Hospital of Gansu University of Chinese Medicine,Baiyin 730900,Gansu Province,China)

机构地区：[1]甘肃中医药大学公共卫生学院,甘肃兰州730000 [2]甘肃中医药大学中医临床学院,甘肃兰州730000 [3]甘肃中医药大学第三附属医院职业病科,甘肃白银730900

出　　处：《中国临床药理学杂志》2024年第23期3405-3409,共5页The Chinese Journal of Clinical Pharmacology

基　　金：甘肃省自然科学基金资助项目(23JRRD0004);甘肃省科技计划基金资助项目(23JDKD0001)。

摘　　要：目的探讨玉屏风散通过肿瘤坏死因子-α(TNF-α)/NOD样受体蛋白3(NLRP3)/胱天蛋白酶1(Caspase-1)/白细胞介素(IL)-1β信号通路对尘肺巨噬细胞的影响。方法用脂多糖复制细胞炎症模型。将Raw264.7巨噬细胞分为正常组(常规培养)、模型组(细胞炎症模型,给予脂多糖处置)、阳性组(1×10^(-7)mol·L^(-1)地塞米松)和低、中、高剂量实验组(0.3、0.6、0.8 g·mL^(-1)含玉屏风散血清处置)。用实时荧光定量聚合酶链反应法检测细胞mRNA表达水平。结果正常组、模型组、阳性组、低剂量实验组、中剂量实验组和高剂量实验组细胞上清液TNF-αmRNA相对表达水平分别为1.00±0.05、2.17±0.07、1.17±0.08、1.90±0.08、1.54±0.07和1.35±0.05,核因子-κB mRNA相对表达水平分别为1.00±0.04、2.24±0.06、1.16±0.06、1.98±0.06、1.65±0.11和1.35±0.03,Caspase-1 mRNA相对表达水平分别为1.00±0.04、1.90±0.03、1.13±0.04、1.73±0.08、1.56±0.06和1.30±0.06,IL-18 mRNA相对表达水平分别为1.00±0.04、1.51±0.04、1.12±0.06、1.41±0.05、1.31±0.06和1.21±0.04,IL-1βmRNA相对表达水平分别为1.00±0.04、1.70±0.08、1.12±0.01、1.52±0.07、1.37±0.04和1.22±0.06,NLRP3 mRNA相对表达水平分别为1.00±0.03、1.90±0.05、1.12±0.05、1.70±0.10、1.50±0.04和1.23±0.06,Gasdermin D蛋白(GSDMD)mRNA相对表达水平分别为1.00±0.06、1.70±0.08、1.11±0.01、1.53±0.05、1.37±0.07和1.22±0.03。模型组的上述指标与正常组比较,低、中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论玉屏风散可以抑制TNF-α/NLRP3/Caspase-1/IL-1β信号通路的激活,减轻肺泡炎症。Objective To explore the effects of Yupingfeng powder on pneumoconiosis macrophage cells through tumor necrosis factor-α/NOD like receptor protein 3/cysteinyl aspartate specific proteinase-1/interleukin-1β(TNF-α/NLRP3/Caspase-1/IL-1β)signaling pathways.Methods Using lipopolysaccharides to replicate a cellular inflammatory model.Raw264.7 macrophage cells were divided into normal group(conventional culture),model group(cellular inflammation model,administer lipopolysaccharide treatment),positive group(1×10-7 mol·L^(-1) dexamethasone)and experimental-L,-M,-H groups(0.3,0.6,0.8 g·mL^(-1) Yupingfeng powder containing serum).Real time fluorescence quantitative polymerase chain reaction was used to detect the exp re ssion of mRNA in each group of cells.Results The relative expression levels of TNF-αmRNA in normal group,model group,positive group and experimental-L,-M,-H groups were 1.00±0.05,2.17±0.07,1.17±0.08,1.90±0.08,1.54±0.07 and 1.35±0.05;the relative expression levels of nuclear factor-κB mRNA were 1.00±0.04,2.24±0.06,1.16±0.06,1.98±0.06,1.65±0.11 and 1.35±0.03;the relative expression levels of Caspase-1 mRNA were 1.00±0.04,1.90±0.03,1.13±0.04,1.73±0.08,1.56±0.06 and 1.30±0.06;the relative expression levels of IL-18 mRNA were 1.00±0.04,1.51±0.04,1.12±0.06,1.41±0.05,1.31±0.06 and 1.21±0.04;the relative expression levels of IL-1βmRNA were 1.00±0.04,1.70±0.08,1.12±0.01,1.52±0.07,1.37±0.04 and 1.22±0.06;the relative expression levels of NLRP3 mRNA were 1.00±0.03,1.90±0.05,1.12±0.05,1.70±0.10,1.50±0.04 and 1.23±0.06;the relative expression levels of Gasdermin D(GSDMD)mRNA were 1.00±0.06,1.70±0.08,1.11±0.01,1.53±0.05,1.37±0.07 and 1.22±0.03.The above indicators in the model group showed statistically significant differences compared to the normal group(P<0.05,P<0.01);there were statistical differences between the above indicators of experimental-L,-M,-H groups and model group(P<0.05,P<0.01).Conclusion Yupingfeng powder can inhibit the activation of the TNF-α/NLRP3/Ca

关 键 词：玉屏风散 肿瘤坏死因子-α/NOD样受体蛋白3/胱天蛋白酶1/白细胞介素-1β信号通路 RAW264.7巨噬细胞 脂多糖 

分 类 号：R28[医药卫生—中药学]