氢溴酸樟柳碱对运动性疲劳大鼠心肌氧化应激性损伤的影响  

Effects of anisodine hydrobromide on myocardial oxidative stress injury in rats with exercise-induced fatigue

作　　者：王训令[1] 邱雷 孙慧 WANG Xun-ling;QIU Lei;SUN Hui(School of Physical Education,Huaibei Normal University,Huaibei 235000,Anhui Province,China;Information College,Huaibei Normal University,Huaibei 235000,Anhui Province,China;Cardiology Department of Huaibei Traditional Chinese Medicine Hospital,Huaibei 235000,Anhui Province,China)

机构地区：[1]淮北师范大学体育学院,安徽淮北235000 [2]淮北师范大学信息学院,安徽淮北235000 [3]淮北市中医医院心病科,安徽淮北235000

出　　处：《中国临床药理学杂志》2024年第23期3434-3438,共5页The Chinese Journal of Clinical Pharmacology

基　　金：安徽省高校人文社科研究基金资助项目(SK2020A0980)。

摘　　要：目的考察氢溴酸樟柳碱对运动性疲劳大鼠心肌氧化应激性损伤的影响。方法将大鼠分为对照组、模型组和低、中、高剂量实验组。对照组和模型组均灌胃等量0.9%NaCl;低、中、高剂量实验组分别灌胃0.3、0.6和1.2 mg·kg^(-1)氢溴酸樟柳碱溶液。5组大鼠均连续给药4周。除对照组之外,其他4组大鼠给药2 h后,用负重力竭游泳运动构建运动性疲劳大鼠模型,共造模4周。用微量法检测血清乳酸脱氢酶(LDH)水平,用TUNEL染色法评价心肌细胞凋亡情况,用微量法检测心肌组织超氧化物歧化酶(SOD)活性,用蛋白质印迹法检测心肌组织中细胞核核因子E2相关因子2(Nrf2)蛋白的相对表达水平。结果对照组、模型组和低、中和高剂量实验组的血清LDH水平分别为(991.79±123.88)、(2983.06±281.28)、(2309.06±271.81)、(1856.17±214.16)和(1380.29±189.81)U·L^(-1),心肌TUNEL阳性率分别为(5.09±0.42)%、(48.43±5.57)%、(30.97±3.43)%、(21.57±2.31)%和(9.49±1.36)%,心肌SOD活性分别为(102.06±8.96)、(59.65±7.66)、(73.25±7.60)、(80.78±6.22)和(84.76±8.94)U·mg prot^(-1),细胞核Nrf2蛋白相对表达水平分别为1.00±0.08、0.31±0.03、0.60±0.05、0.89±0.06和1.21±0.09。模型组的上述指标与对照组比较,低、中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论氢溴酸樟柳碱能够减轻运动性疲劳大鼠心肌氧化应激性损伤和细胞凋亡,其机制与激活Nrf2介导的抗氧化途径有关。Objective To investigate the effect of anisodine hydrobromide on myocardial oxidative stress injury in rats with exercise-induced fatigue.Methods The rats were divided into control group,model group and experimental-L,-M,-H groups.The rats in control group and model group were intragastrically infused with 0.9%NaCl.The rats in experimental-L,-M,-H groups were fed with 0.3,0.6 and 1.2 mg·kg^(-1)·d^(-1) anisodine hydrobromide solution,respectively.The rats in five groups were all given the drug for four consecutive weeks.Except for control group,the rats in other four groups were subjected to weight-bearing exhaustive swimming exercise to establish the exercise-induced fatigue rat model 2 hours after drug administration,and the model was established for a total of four weeks.The levels of serum lactate deh yd rogenase(LDH)was measured by micromethod,the apoptosis of cardiomyocytes was evaluated by TUNEL staining,the activity of superoxide dismutase(SOD)was detected by micromethod,and the expression of nuclear factor E2 related factor 2(Nrf2)protein was detected by Western blot.Results The serum LDH levels of control group,model group and experimental-L,-M,-H groups were(991.79±123.88),(2983.06±281.28),(2309.06±271.81),(1856.17±214.16)and(1380.29±189.81)U·L^(-1),respectively;the positive rates of myocardial TUNEL were(5.09±0.42)%,(48.43±5.57)%,(30.97±3.43)%,(21.57±2.31)%and(9.49±1.36)%,respectively;the SOD activities of myocardium were(102.06±8.96),(59.65±7.66),(73.25±7.60),(80.78±6.22)and(84.76±8.94)U·mg prot-1,respectively;the relative expression levels of nuclear Nrf2 protein were 1.00±0.08,0.31±0.03,0.60±0.05,0.89±0.06 and 1.21±0.09,respectively.The above indicators in the model group were compared with control group,and the above indicators in the experimental-L,-M,-H groups were compared with model group,and the differences were statistically significant(all P<0.05).Conclusion Anisodine hydrobromide can reduce the oxidative stress injury and apoptosis of cardiac muscle in rats with exer

关 键 词：氢溴酸樟柳碱 运动性疲劳 氧化应激 细胞凋亡 核因子E2相关因子2 

分 类 号：R972[医药卫生—药品]