Self‑Assembling Peptide Nanofibers Anchored Parathyroid Hormone Derivative for Bone Tissue Engineering  

作　　者：Zhuowen Hao Tianhong Chen Ying Wang Qinyu Feng Jiayao Chen Hanke Li Junwu Wang Zepu Wang Zheyuan Zhang Renxin Chen Guang Shi Zhenwei Zou Lin Cai Tonghe Zhu Jingfeng Li 

机构地区：[1]Department of Orthopedics,Zhongnan Hospital of Wuhan University,Wuhan 430071,China [2]Frontier Institute of Medical&Pharmaceutical Science and Technology,School of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,China [3]Department of Obstetrics and Gynecology,Renmin Hospital of Wuhan University,Wuhan 430060,China [4]Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [5]Institute of Radiation Oncology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

出　　处：《Advanced Fiber Materials》2024年第2期583-606,共24页先进纤维材料（英文）

基　　金：supported by the National Natural Science Foundation of China(No:82372405,81871752,81874232);the Key Research and Development Program of Hubei Province(No:2022BCA052);the Key Research and Development Program of Wuhan City(No:2023020402010591),and the Fundamental Research Funds for the Central Universities(No:2042023kf0199);the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(No:ZNJC202014).

摘　　要：Parathyroid hormone(PTH)has been used for bone regeneration through intermittent subcutaneous injection;however,the topical administration of PTH for bone repair remains challenging because of the overactivation of osteoclasts.Here,a PTH derivative,i.e.,PTHrP-1,which exhibits enhanced osteogenesis and relatively reduced osteoclastogenesis,is anchored to RADA16-I to fabricate a novel self-assembling peptide,called P1R16.Firstly,P1R16 self-assembles into long nanofibers with PTHrP-1 exposed to the side end,which interacts with Type I collagen(Col)to form P1R16-Col composites.The RADA16 segment in P1R16 helps the sustained release of P1R16 from the composites.Secondly,the P1R16 self-assembling peptide nanofibers exhibit multiple functions.The nanofibers promote stem cell proliferation and recruitment,and then direct stem cell fate towards osteogenic differentiation but not adpipogenic differentiation,improving the quality of the regenerated bone.The nanofibers further promote bone regeneration through bone remodeling between osteoblasts and osteoclasts.Thirdly,the P1R16 self-assembling peptide nanofibers also promote the proliferation and recruitment of endothelial cells,which facilitate the vascularization of implants to support bone regeneration further.Overall,the P1R16 self-assembling peptide nanofibers maintain multiple functions,including pro-proliferation,direction of stem cell fate,bone remodeling and vascularization,showing considerable promise for bone tissue engineering to repair bone defects or fractures.

关 键 词：Self-assembling peptide NANOFIBER Parathyroid hormone Bioactive factor Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]