3D Printing-Electrospinning Hybrid Nanofibrous Scaffold as LEGO-Like Bricks for Modular Assembling Skeletal Muscle-on-a-Chip Functional Platform  

作　　者：Zihan Wang Sitian Liu Mingying Han Jie Xu Maoyu Qin Qiao Yang Guanjie Zeng Meng Long Ting Li Junfeiyang Yin Liu Yu Wenhua Huang Ling Wang Yaobin Wu 

机构地区：[1]Guangdong Engineering Research Center for Translation of Medical 3D Printing Application,Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics,Department of Human Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,China [2]Biomaterials Research Center,School of Biomedical Engineering,Southern Medical University,Guangzhou 510515,China [3]Department of General Surgery,Nanfang Hospital,First Clinical Medical College,Southern Medical University,Guangzhou 510515,China [4]Department of Spinal Surgery,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China

出　　处：《Advanced Fiber Materials》2024年第5期1521-1540,共20页先进纤维材料（英文）

基　　金：supported by the National Natural Science Foundation of China(32271423,32000955);the National Key R&D Program of China(2022YFB4600600);the Guangdong Basic and Applied Basic Research Foundation(2024A1515013273).

摘　　要：Organ-on-a-chip stands as a pivotal platform for skeletal muscle research while constructing 3D skeletal muscle tissues that possess both macroscopic and microscopic structures remains a considerable challenge.This study draws inspiration from LEGO-like assembly,employing a modular approach to construct muscle tissue that integrates biomimetic macroscopic and microscopic structures.Modular LEGO-like hybrid nanofibrous scaffold bricks were fabricated by the combination of 3D printing and electrospinning techniques.Skeletal muscle cells cultured on these modular scaffold bricks exhibited a highly orientated nanofibrous structure.A variety of construction of skeletal muscle tissues further enabled development by various assembling processes.Moreover,skeletal muscle-on-a-chip(SMoC)was further assembled as a functional platform for electrical or perfusion stimuli investigation.The electrical stimulus was conveniently applied and tuned in such a SMoC platform to significantly enhance the differentiation of skeletal muscle tissues.Additionally,the effect of perfusion stimulation on skeletal muscle vascularization within the SMoC platform was also demonstrated.These findings highlight the potential of these assembled SMoCs as functional ex vivo platforms for skeletal tissue engineering and drug research applications,and such a LEGO-like assembly strategy could also be applied to the other engineering organ-on-chips fabrication,which facilitates the development of bionic functional platforms for various biomedical research applications.

关 键 词：3D printing ELECTROSPINNING LEGO assembly Skeletal muscle-on-a-chip Vascular networks 

分 类 号：R318[医药卫生—生物医学工程] TB383[医药卫生—基础医学]