Synergistic microcystin degradation by a novel bacterium isolated from shrimp pond and fulvic acids  

作　　者：Genghua QIN Wei DAI Xiangdong BI Jiang WU Weilin RUAN Yanzhao WANG 

机构地区：[1]Key Laboratory of Aquatic-Ecology and Aquaculture of Tianjin,Department of Fisheries Sciences,Tianjin Agricultural University,Tianjin 300384,China

出　　处：《Journal of Oceanology and Limnology》2024年第6期1817-1826,共10页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.32172978,31772857);the Natural Science Foundation of Tianjin(No.22JCYBJC00430);the Tianjin Science and Technology Planning Project(No.22ZYCGSN00050);the Fundamental Research Funds of Tianjin Universities(Nos.2020ZD06,2021KJ110);the Gansu Science and Technology Project(Nos.21CX6NP223,2023ZZ1NC006)。

摘　　要：Using the Widdel medium with extracted microcystin(MC)as the sole carbon and nitrogen sources,the MC-degrading bacteria community S_6 was enriched from the sediment of Litopenaeus vannamei pond,and a novel MC-degrading bacteria strain was isolated from S_6.According to 16S rDNA gene sequence and biochemical characteristics,the isolated strain was identified and named Nitratireductor aquimarinus D_(1).Fulvic acid(FA),as a widely existing photosensitizer involved in MC photodegradation,coexists with MC-degrading bacteria in natural water.The synergistic effects of N.aquimarinus D_(1) and FA on MC degradation were evaluated via comparing the degradation rate of MC induced by N.aquimarinus D_(1) and FA alone and in combination under natural light conditions.Compared with the control group,the supplementation of N.aquimarinus D_(1) and FA alone or in combination could significantly increase the degradation rate of MC(P<0.05).In the first 36 h,the degradation effect of FA on MC was better than that of N.aquimarinus D_(1),but the degradation effect was opposite at 48 h.N.aquimarinus D_(1) and FA did not show synergistic effect on MC degradation until 48 h.In the application of N.aquimarinus and FA to degrade MC in aquaculture pond,there might be a time-lag effect in the synergistic degradation.

关 键 词：synergistic degradation MICROCYSTIN N.aquimarinus D_1 fulvic acid 

分 类 号：X714[环境科学与工程—环境工程]