Effects of light spectra on growth and compositions of biomass,fatty acids,and pigments in three typical microalgae from different phyla  

作　　者：Fei KONG Jiaxing ZHANG Dongzi YANG Yao ZHOU Yanrong LI Deshui CHEN Xiaojun YAN Zhaoshou RAN Jilin XU 

机构地区：[1]Key Laboratory of Aquacultural Biotechnology Ministry of Education,Ningbo University,Ningbo 315211,China [2]Ningbo Institute of Oceanography,Ningbo 315832,China [3]Key Laboratory of Marine Biotechnology of Zhejiang Province,Ningbo 315211,China [4]Fujian Dalai Seedling Technology Co.,Ltd.,Luoyuan 350600,China

出　　处：《Journal of Oceanology and Limnology》2024年第6期1976-1990,共15页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.32102763);the Ningbo Science and Technology Research Projects;China(No.2019B10006);the earmarked fund(No.CARS-49);the Ningbo Science and Technology Research Projects;China(No.2024Z276)。

摘　　要：The growth and nutrients of photosynthetic organisms are significantly influenced by light spectra,yet this relationship is not well-understood in microalgae.Herein,we studied three microalgae species—Chaetoceros sp.,Isochrysis galbana,and Tetraselmis helgolandica—distinguished by their pigments.We exposed them to seven light spectra,including white(control),red,orange,green,blue,violet,and full spectrum.The results showed distinct responses in the three microalgae to varying light spectra.Optimal growth occurred under blue,violet,and white lights for Chaetoceros sp.,Ⅰ.galbana,and T.helgolandica,respectively,while orange,red,and green lights inhibited growth.Notably,green light significantly increased the protein content in all three microalgae.Carbohydrate and lipid content exhibited species-specific responses:the highest carbohydrate accumulation was achieved for Chaetoceros sp.under red light(73.27±1.45 mg/g),Ⅰ.galbana under orange light(122.89±12.28 mg/g),and T.helgolandica under blue light(43.62±2.79 mg/g).Meanwhile,the highest lipid content was obtained under violet light for Chaetoceros sp.andⅠ.galbana(250.80±7.27 and 320.23±5.75 mg/g,respectively),and under green light for T.helgolandica(255.12±31.19 mg/g).Furthermore,violet light greatly promoted the accumulation of polyunsaturated fatty acids in all three microalgae.Specific pigment compositions also responded to variations in light spectra.For instance,the diadinoxanthin content in Chaetoceros sp.increased significantly under orange light(194.77±13.78μg/g),while chlorophyll-a content inⅠ.galbana increased significantly under violet and blue lights((88.84±33.46)-(141.38±1.64)μg/g),and in T.helgolandica under red,green,and blue lights((1485.04±190.46)-(1886.60±387.42)μg/g).Additionally,Ⅰ.galbana exhibited the highest fucoxanthin,diadinoxanthin,andβ-carotene contents under white light.In conclusion,our results highlight the species-specific impact of light spectra on microalgae growth and nutrients,providing valuable guidance

关 键 词：MICROALGAE light spectrum GROWTH biomass composition fatty acids PIGMENT 

分 类 号：Q948.8[生物学—植物学]