Feeding time changed the diurnal composition of intestinal microbiota in Pelteobagrus fulvidraco  

作　　者：Chuanjie QIN Jing XU Quan GONG Zhengyong WEN Yi LIU Qingchao SHI 

机构地区：[1]Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River,Neijiang Normal University,Neijiang 641000,China [2]College of Life Science,Neijiang Normal University,Neijiang 641000,China [3]Fisheries Research Institute‚Sichuan Academy of Agricultural Sciences,Chengdu 611730,China

出　　处：《Journal of Oceanology and Limnology》2024年第6期2034-2044,共11页海洋湖沼学报（英文）

基　　金：Supported by the Sichuan Science and Technology Program(Nos.2021YFYZ0015,2023NSFSC0241)。

摘　　要：Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was conducted.Two triplicate groups of fish were fed identically either at 8:00(day)or at 20:00(night).After 9 weeks,three fish from each cage were sacrificed every 4 h for 24 h.The intestinal contents were analyzed using high-throughput sequencing.Three phyla(Fusobacteria,Proteobacteria,and Verrucomicrobia),and three genera(Cetobacterium,Akkermansia,and Clostridium)were dominant at every time point.Moreover,33.02%of detected amplicon sequence variants showed cyclical variations in fish fed at 20:00,and 3.47%in fish fed at 8:00.Among the top 20 genera,17 showed cyclical variations in the 20:00 group,and only two in the 8:00 group.Rhodobacter,Ralstonia,Mycobacterium,Lactococcus,Halomonas,Akkermansia,and Acinetobacter contributed more to the predicted functional pathways.Moreover,144 and 38 pathways exhibited daily cyclical fluctuations in fish fed at 20:00 and 8:00,respectively.Overall,certain intestinal microorganisms showed a circadian rhythm in P.fulvidraco,and this circadian rhythm was affected by shifted feeding times.

关 键 词：diurnal fluctuation Pelteobagrus fulvidraco gut microorganism feeding time 

分 类 号：S965.199[农业科学—水产养殖]