Betaine combined with traditional Chinese medicine ointment to treat skin wounds in microbially infected diabetic mice  

作　　者：Wen-Yan Xu Yuan-Yuan Dai Shi-Xian Yang Hao Chen Yan-Qiang Huang Pei-Pei Luo Zhong-Heng Wei 

机构地区：[1]Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance,Youjiang Medical University for Nationalities,Baise 533000,Guangxi Zhuang Autonomous Region,China [2]Guangxi Clinical Medical Research Center for Hepatobiliary Diseases,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi Zhuang Autonomous Region,China [3]Department of Pathology,Wannan Medical College,Wuhu 241002,Anhui Province,China [4]Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areas,Youjiang Medical University for Nationalities,Baise 533000,Guangxi Zhuang Autonomous Region,China [5]Department of Gastroenterology,Wujin People’s Hospital Affiliated to Jiangsu University,Changzhou 213004,Jiangsu Province,China

出　　处：《World Journal of Diabetes》2025年第1期104-120,共17页世界糖尿病杂志（英文）

基　　金：Supported by Guangxi Science and Technology Major Projects,No.2023AA20009;National Natural Science Foundation of China,No.32360035 and No.32060018.

摘　　要：BACKGROUND Skin wounds are highly common in diabetic patients,and with increasing types of pathogenic bacteria and antibiotic resistance,wounds and infections in diabetic patients are difficult to treat and heal.AIM To explore the effects of betaine ointment(BO)in promoting the healing of skin wounds and reducing the inflammation and apoptosis of skin cells in microbially infected diabetic mice.METHODS By detecting the minimum inhibitory concentrations(MICs)of betaine and plant monomer components such as psoralen,we prepared BO with betaine as the main ingredient,blended it with traditional Chinese medicines such as gromwell root and psoralen,and evaluated its antibacterial effects and safety in vitro and in vivo.The skin infection wound models of ordinary mice and diabetic mice were constructed,and the OTC drugs mupirocin ointment and Zicao ointment were used as controls to evaluate the antibacterial effects in vivo and the anti-inflammatory and anti-apoptotic effects of BO.RESULTS The MICs of betaine against microorganisms such as Staphylococcus aureus(S.aureus),Candida albicans and Cryptococcus neoformans ranged from 4 to 32μg/mL.Gromwell root and psoralea,both of which contain antimicrobial components,mixed to prepare BO with MICs ranging from 16 to 64μg/mL,which is 32-256 times lower than those of Zicao ointment,although the MIC is greater than that of betaine.After 15 days of treatment with BO for USA300-infected ordinary mice,the wound scab removal rates were 83.3%,while those of mupirocin ointment and Zicao ointment were 66.7%and 0%,respectively,and the differences were statistically significant.In diabetic mice,the wound scab removal rate of BO and mupirolacin ointment was 80.0%,but BO reduced wound inflammation and the apoptosis of skin cells and facilitated wound healing.CONCLUSION The ointment prepared by mixing betaine and traditional Chinese medicine can effectively inhibit common skin microorganisms and has a strong effect on the skin wounds of sensitive or drug-resistant S.aureus-infected ordina

关 键 词：Betaine ointment Staphylococcus aureus Drug resistance Skin infection Diabetes mellitus 

分 类 号：R587.1[医药卫生—内分泌]