柳叶白前不同蜜制方法对体外溶出度的影响研究  

Study on the Influence of Different Honey Preparation Methods on Dissolution of Willow Leaf Baiqian in vitro

作　　者：薛春龙 王雅婷 张玺雅 董征艳 王伊楠 郭宇航 段秀俊 XUE Chunlong;WANG Yating;ZHANG Xiya;DONG Zhengyan;WANG Yinan;GUO Yuhang;DUAN Xiujun(Laboratory of Traditional Chinese Medicine Pharmaceutics,Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西中医药大学中药药剂学实验室,晋中030619

出　　处：《世界科学技术-中医药现代化》2024年第9期2415-2424,共10页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：山西省科学技术厅2016年度山西省重点研发计划中药现代化振东专项基金(2016ZD0201):中药配方颗粒开发研究,负责人:裴妙荣。

摘　　要：目的建立柳叶白前质量标准,评价不同蜜炙方法对柳叶白前体外溶出度的影响。方法采用高效液相色谱法。(1)胡萝卜苷和β-谷甾醇含量测定方法:色谱柱:Dia-monsil C18(250 mm×4.6 mm,5μm);流动相:甲醇:0.05%磷酸水(99∶1)。等度洗脱;流速1.0 mL·min^(-1);检测波长209 nm;进样量10μL;柱温30℃。(2)葡萄糖果糖测定方法:色谱柱为Inspire NH2糖分析专用柱(250 mm×4.6 mm,5μm);以乙腈-水(77:23)为流动相;流速为0.8 mL·min^(-1);柱温为35℃;进样量10μL;蒸发光散射检测器(ELSD):漂移管温度为100℃,载气流速为2.8 L·min^(-1)。结果(1)结果显示胡萝卜苷和β-谷甾醇成分的测定在质量浓度范围内呈现良好的线性关系(r>0.9999),平均加样回收率为99.71%和98.83%,精密度、稳定性、重复性RSD均小于2.0%。(2)葡萄糖果糖建立的方法在0.05-0.5 mg·mL^(-1)(r=0.9991)范围内,呈良好的线性关系。结论该研究含量测定方法及体外溶出度实验方法学考察结果符合要求,为制定蜜炙柳叶白前质量标准和现代研究提供了科学依据。Objective To evaluate the effect of different honey processing methods on the dissolution of Cynanchum salicifolia in vitro.Methods High performance liquid chromatography①Carotene andβ-Determination method of sitosterol content:chromatographic column:Dia monosil C18(250 mm×4.6 mm,5μm).Mobile phase:methanol:0.05%phosphoric acid water=99∶1.Isocratic elution;Flow rate 1.0 mL·min^(-1);Detection wavelength 209 nm;Injection volume 10μL.The column temperature is 30℃.②Determination method of glucose and fructose:the chromatographic column is Inspire NH2 sugar analysiscolumn(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile water(77∶23).The flow rate is 0.8 mL·min^(-1);The column temperature is 35℃;Injection volume 10μL;Evaporative light scattering detector(ELSD):the drift tube temperature is 100℃,and the carrier gas flow rate is 2.8 L·min^(-1).Results①The results showed that carotene andβ-the determination of sitosterol showed a good linear relationship in the range of mass concentration(r>0.9999).The average recoveries were 99.71%and 98.83%.The RSD of precision,stability and repeatability were all less than 2.0%.②The established method of glucose fructose showed a good linear relationship in the range of 0.05-0.50 mg·mL-1(r=0.9991).Conclusion The content determination method and in vitro dissolution test methodology of the study meet the requirements,which provides a scientific basis for the establishment of quality standards and modern research of honey roasted Paeonia salicifolia.

关 键 词：柳叶白前 胡萝卜苷 Β-谷甾醇 葡萄糖 果糖 

分 类 号：R283.6[医药卫生—中药学]