先天性心脏病合并肺动脉高压患儿血清P2X7R的表达及意义  

Expression and significance of P2X7R in serum of children with congenital heart disease and pulmonary hypertension

作　　者：王文静 范双 颜松 WANG Wenjing;FAN Shuang;YAN Song(Department of Pediatrics,Dazu Hospital Affiliated to Chongqing Medical University(Chongqing Dazu District People's Hospital),Chongqing 402360,China)

机构地区：[1]重庆医科大学附属大足医院/重庆市大足区人民医院儿科,重庆402360

出　　处：《中国急救复苏与灾害医学杂志》2024年第12期1622-1625,共4页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：重庆市大足区2022年科卫联合医学科研项目(编号:DZKJ2022JSYJ-KWXM1031)。

摘　　要：目的探讨先天性心脏病(CHD)合并肺动脉高压(PAH)患儿血清嘌呤受体P2X配体门控性离子通道7(P2X7R)的表达及意义。方法选取2020年6月—2022年12月本院收治的80例CHD患儿,其中单纯CHD 18例,合并轻度PAH 26例,中度20例,重度16例;选取20例健康儿童为对照组。用酶联免疫吸附试验检测血清P2X7R表达水平。观察缺氧对人肺血管平滑肌细胞P2X7R蛋白表达的影响;观察沉默P2X7R基因对缺氧人肺血管平滑肌细胞增殖和迁移的影响。结果CHD合并PAH患儿血清P2X7R水平高于健康儿童,并且随着PAH病情加重,血清P2X7R表达水平升高(P<0.05)。缺氧使人肺血管平滑肌细胞P2X7R蛋白表达水平下降(P<0.05)。沉默P2X7R基因使缺氧人肺血管平滑肌细胞的增殖和迁移力降低(P<0.05)。结论CHD合并PAH患儿血清P2X7R水平升高,P2X7R可能通过影响肺血管平滑肌细胞增殖和迁移参与PAH发生发展。Objective To investigate the expression and significance of purine receptor P2X ligand gated ion channel 7(P2X7R)in children with congenital heart disease(CHD)and pulmonary hypertension(PAH).Methods 80 children with CHD admitted to our hospital from June 2020 to December 2022 were seleceted,including 18 cases of simple CHD,6 cases of CHD combined with mild PAH,20 cases of moderate PAH,and 16 cases of severe PAH.20 healthy children were selected as controls.The expression level of serum P2X7R using enzyme-linked immunosorbent assay was detected.The effect of hypoxia on the expression of P2X7R protein in human pulmonary vascular smooth muscle cells,the effect of silencing the P2X7R gene on the proliferation and migration of hypoxic human pulmonary vascular smooth muscle cells were observed.Results The serum P2X7R level in children with CHD combined with PAH was higher than those in healthy children,and as the condition of PAH worsened,the expression level of serum P2X7R was higher(P<0.05).Hypoxia reduced the expression level of P2X7R protein in human pulmonary vascular smooth muscle cells(P<0.05).Silencing the P2X7R gene reduced the proliferation and migration of hypoxic human pulmonary vascular smooth muscle cells(P<0.05).Conclusion The level of P2X7R in serum of children with CHD and PAH increased,and P2X7R may participate in the development of PAH by affecting the proliferation and migration of pulmonary vascular smooth muscle cells.

关 键 词：先天性心脏病 肺动脉高压 嘌呤受体P2X配体门控性离子通道7 增殖 迁移 

分 类 号：R725.4[医药卫生—儿科]