一种具有激发态分子内质子转移特性的荧光小分子在铜离子检测及细胞成像中的应用  

A Fluorescent Small Molecule with Excited State Intramolecular Proton Transfer Property for Detection of Cu^(2+) and Cellular Imaging

作　　者：张岩 卢煜松 刘吴颖 曹颖 王丽娜 ZHANG Yan;LU Yu-Song;LIU Wu-Ying;CAO Ying;WANG Li-Na(Chongqing Key Laboratory of Green Synthesis and Applications,College of Chemistry,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学化学学院,绿色合成与应用重庆市重点实验室,重庆401331

出　　处：《分析化学》2024年第11期1726-1734,共9页Chinese Journal of Analytical Chemistry

基　　金：重庆市教委科技项目(No.KJQN202100502)资助。

摘　　要：荧光传感技术在快速检测和可视化分析研究中发挥了关键作用。本研究采用两步合成法制备了一种新型荧光小分子SA-AP-PPh_(3)。此荧光小分子由三苯基膦和水杨醛-苯胺席夫碱组成,具有激发态分子内质子转移(ESIPT)特性和较大的斯托克斯位移,可以选择性检测Cu^(2+),检测范围为0.5~15μmol/L,检出限低至261 nmol/L,可用于实际水样中Cu^(2+)浓度的测定。此荧光小分子具备线粒体靶向功能,能够实现细胞线粒体的可视化成像,可用于细胞内Cu^(2+)的检测。Fluorescent sensing technology plays a crucial role in rapid detection of substances and research onvisual analysis. In this work, a new type of fluorescent molecule, named SA-AP-PPh3, was successfully synthesizedthrough a two-step process, with a structure composed of triphenylphosphine and salicylaldehyde-aniline Schiffbase. This molecule showed the nature of excited-state intramolecular proton transfer (ESIPT) and a large Stokesshift. SA-AP-PPh3 had the ability to selectively detect Cu^(2+), with a detection range from 0.5 to 15 μmol/L and adetection limit as low as 261 nmol/L, which could be used to determine the concentration of Cu^(2+) in actual watersamples. In addition, the molecule contained a mitochondrial targeting functional group and had goodbiocompatibility, which could achieve the visualization of cellular mitochondria and effective detectionof Cu^(2+) in cells.

关 键 词：聚集诱导发光 激发态分子内质子转移 铜离子 检测 细胞成像 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]