多重耐药鼠伤寒沙门菌菌株耐药基因及毒力基因的研究分析  

Distribution of resistance genes and virulence genes in multidrug-resistant Salmonella typhimurium strains

作　　者：张玉凤 宋鹤 闫乐 徐鹏飞 刘瑞清 唐甜甜[1] 王小燕[1] 邓慧玲 雷铠睿 Zhang Yufeng;Song He;Yan Le;Xu Pengfei;Liu Ruiqing;Tang Tiantian;Wang Xiaoyan;Deng Huiling;Lei Kairui(Department of Infectious Diseases,Children's Hospital Affiliated to Xi'an Jiaotong University,Xi'an 710002,China;School of Public Health,Shaanxi University of Traditional Chinese Medicine,Xi'an 712046,China)

机构地区：[1]西安交通大学附属儿童医院感染二科,710002 [2]陕西中医药大学公共卫生学院,西安712046

出　　处：《中国小儿急救医学》2024年第11期831-835,共5页Chinese Pediatric Emergency Medicine

基　　金：陕西省重点研发计划项目(2022SF-142)。

摘　　要：目的研究多重耐药鼠伤寒沙门菌菌株耐药基因和毒力基因。方法收集鼠伤寒沙门菌菌株共96株,进行药敏试验,评价鼠伤寒沙门菌的耐药性,记录鼠伤寒沙门菌的多重耐药情况。选取多重耐药鼠伤寒沙门菌菌株进行全基因组测序,分析菌株的耐药基因和毒力基因分布。结果鼠伤寒沙门菌菌株对氨苄西林、氨苄西林/舒巴坦的耐药率最高,分别为89.58%、76.04%,其次为甲氧苄啶/磺胺甲噁唑、头孢曲松、氨曲南,分别为47.92%、38.54%、33.33%,对环丙沙星、左氧氟沙星的耐药率较低,分别为8.33%、4.17%,96株菌株对碳青霉烯类抗生素及哌拉西林/他唑巴坦均敏感。57株(59.38%)鼠伤寒沙门菌菌株表现为多重耐药。57株多重耐药鼠伤寒沙门菌菌株均检测出耐药基因,其中以aac(6')-Iaa、aadA22、blaTEM-1B的携带率较高,分别为98.25%、77.19%、59.65%。多重耐药鼠伤寒沙门菌菌株对invA、sipA、sseL、sopB的携带率最高。结论鼠伤寒沙门菌菌株多重耐药发生率高,对多种耐药基因和毒力基因的携带率高,临床中应加强对鼠伤寒沙门菌的监测和防控,以减少多重耐药菌株的播散流行。ObjectiveTo study the distribution of drug resistance genes and virulence genes in multidrug-resistant Salmonella typhimurium strains.MethodsA total of 96 strains of Salmonella typhimurium were collected,and drug sensitivity tests were performed to evaluate the drug resistance and multidrug-resistance of Salmonella typhimurium.Multidrug-resistant Salmonella typhimurium strains were selected to conducted whole genome sequencing,and the distribution of drug resistance genes and virulence genes in the strain were analyzed.ResultsSalmonella typhimurium strains had the highest resistance rates to ampicillin and ampicillin/sulbactam,with 89.58%and 76.04%,respectively.Followed by trimethoprim/sulfamethoxazole,ceftriaxone,and aztreonam,with 47.92%,38.54% and 33.33%,respectively,and low resistance rates to ciprofloxacin and levofloxacin,with 8.33% and 4.17%,respectively.Ninety-six strains were all sensitive to carbapenem antibiotics and piperacillin/tazobactam.Fifty-seven strains(59.38%)of Salmonella typhimurium showed multidrug-resistance.Resistance genes were detected in all 57 multidrug-resistant Salmonella typhimurium strains,with higher carrier rates of 98.25%,77.19%,and 59.65% for aac(6')-Iaa,aadA22,and blaTEM-1B,respectively.The multidrug-resistant Salmonella typhimurium strains had the highest carrier rates for invA,sipA,sseL,and sopB.ConclusionMultidrug-resistant Salmonella typhimurium strains have a high incidence and a high carrier rate for multiple drug resistance genes and virulence genes.The monitoring and prevention of Salmonella typhimurium should be strengthened in the clinic in order to reduce the spreading epidemic of multidrug-resistant strains.

关 键 词：多重耐药 鼠伤寒沙门菌 耐药基因 毒力基因 

分 类 号：R446.5[医药卫生—诊断学]