西伯利亚白刺盐胁迫响应NsCIPKs基因鉴定及表达分析  

Identification and Expression Level Analysis of Nitraria sibirica NsCIPKs in Response to Salt Stress

作　　者：黄安瀛 燕青 杨秀艳 张会龙[1,2] 张华新 HUANG Anying;YAN Qing;YANG Xiuyan;ZHANG Huilong;ZHANG Huaxin(The Comprehensive Experimental Center of Chinese Academy of Forestry in Yellow River Delta,Dongying Shandong 257500,China;Institute of Ecological Conservation and Restoration,Chinese Academy of Forestry,Beijing 100091,China;Research Institute of Fast-growing Trees,Chinese Academy of Forestry,Zhanjiang Guangdong 524300,China;Zhanjiang Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang Guangdong 524013,China)

机构地区：[1]中国林业科学研究院黄河三角洲综合试验中心,山东东营257500 [2]中国林业科学研究院生态保护与修复研究所,北京100091 [3]中国林业科学研究院速生树木研究所,广东湛江524300 [4]中国热带农业科学院湛江实验站,广东湛江524013

出　　处：《西北农业学报》2024年第12期2337-2346,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：黄河三角洲学者专项经费资助项目(2020);国家自然科学基金(31770640)。

摘　　要：钙离子作为普遍存在的第二信使能够将外界胁迫快速转化为信号传导到细胞内,使植物对胁迫做出响应,而钙调磷酸酶B互作蛋白激酶(CBL interactingprotein kinase,CIPKs)是钙离子信号传导途径的核心和关键之一。本研究以盐生植物西伯利亚白刺(Nitraria sibirica)作为试验材料,基于盐胁迫转录组,利用生物信息学方法对NsCIPKs家族盐胁迫相关基因进行鉴定,采用实时荧光定量PCR(qRT-PCR)分析盐胁迫响应NsCIPKs基因的表达模式。结果表明:共鉴定到8个响应盐胁迫的NsCIPKs成员,均编码碱性亲水蛋白且定位于细胞核,不同基因理化性质存在差异但结构保守。系统进化树分析结果表明:8个NsCIPKs可分为4组,存在于6个亚族中,基因分化均出现于单双子叶植物分化后。qRT-PCR结果显示:NsCIPKs各成员均对不同盐浓度处理的盐胁迫有响应,且集中于根部与茎部表达,根中对盐胁迫响应持久、迅速且高表达的基因为NsCIPK1与NsCIPK7,茎中为NsCIPK3、NsCIPK7与NsCIPK8,叶中为NsCIPK3。综上,NsCIPK1、NsCIPK3、NsCIPK7与NsCIPK8可能在盐胁迫下发挥重要的作用。相关研究结果可为西伯利亚白刺耐盐分子机制探索及转基因新品种创制提供候选基因。Ca^(2+),as a universal second messenger,can quickly transform external stress into signal transduction within cells.The CBL interacting protein kinase(CIPKs)represent a pivotal component in this calcium signaling pathway.The transcriptome of Nitraria sibirica treated with salt stress was used to identify the members of NsCIPKs family in response to salt stress,and verify the expression levels using the real-time fluorescence quantitative PCR(qRT-PCR)method.The results showed the presence of eight NsCIPK genes responsive to salt stress,encoding basic hydrophilic proteins located in the nucleus.Despite differences in their physicochemical properties,these genes displayed conserved structures.Phylogenetic tree analysis classified the eight NsCIPKs into four groups across six subfamilies,indicating that the differentiation of NsCIPKs occurred after the differentiation of monocotyledonous and dicotyledonous plants.Tissue-specific expression analysis indicated a preference for roots and stems under salt stress.NsCIPK1 and NsCIPK7 exhibited high expression levels in roots,indicating a prolonged and rapid response to salt stress,in contrast,NsCIPK3,NsCIPK7,and NsCIPK8 were predominant in stems,while NsCIPK3 showed preferential expression in leaves.This study provides theoretical and practical references for exploration of genes in Nitraria sibirica.

关 键 词：盐胁迫 CIPK 西伯利亚白刺 表达分析 

分 类 号：S793.9[农业科学—林木遗传育种]